{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:14:50Z","timestamp":1774631690527,"version":"3.50.1"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"17","license":[{"start":{"date-parts":[[2021,1,18]],"date-time":"2021-01-18T00:00:00Z","timestamp":1610928000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Swiss National Science Fundation","award":["200021_192274"],"award-info":[{"award-number":["200021_192274"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>Protein immobilization, while widespread to unlock enzyme potential in biocatalysis, remains tied to a trial an error approach. Nonetheless, several databases and computational methods have been developed for protein characterization and their study. CapiPy is a user-friendly application for protein model creation and subsequent analysis with a special focus on the ease of use and interpretation of the results to help the users to make an informed decision on the immobilization approach which should be ideal for a protein of interest. The package has been tested with three separate random sets of 150 protein sequences from Uniprot with more than a 70% overall success rate (see Supplementary information and Supplementary Dataset).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>The package is free to use under the GNU General Public License v3.0. All necessary files can be downloaded from https:\/\/github.com\/drou0302\/CapiPy or https:\/\/pypi.org\/project\/CapiPy\/. All external requirements are also freely available, with some restrictions for non-academic users.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab030","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T00:22:32Z","timestamp":1610497352000},"page":"2761-2762","source":"Crossref","is-referenced-by-count":30,"title":["CapiPy: python-based GUI-application to assist in protein immobilization"],"prefix":"10.1093","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6324-8722","authenticated-orcid":false,"given":"David","family":"Roura Padrosa","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-166X","authenticated-orcid":false,"given":"Valentina","family":"Marchini","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland"}]},{"given":"Francesca","family":"Paradisi","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland"}]}],"member":"286","published-online":{"date-parts":[[2021,1,18]]},"reference":[{"key":"2023051609163412100_btab030-B1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1093\/nar\/28.1.45","article-title":"The SWISS-PROT protein sequence database and its supplement TrEMBL in 2000","volume":"28","author":"Bairoch","year":"2000","journal-title":"Nucleic Acids Res"},{"key":"2023051609163412100_btab030-B2","doi-asserted-by":"crossref","first-page":"D506","DOI":"10.1093\/nar\/gky1049","article-title":"UniProt: a worldwide hub of protein knowledge","volume":"47","author":"Bateman","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2023051609163412100_btab030-B3","doi-asserted-by":"crossref","first-page":"6534","DOI":"10.1039\/c3cs60137d","article-title":"Stabilizing biocatalysts","volume":"42","author":"Bommarius","year":"2013","journal-title":"Chem. 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