{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:40:23Z","timestamp":1762868423415,"version":"3.37.3"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"14","license":[{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["031L0119"],"award-info":[{"award-number":["031L0119"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>An important goal of concentration\u2013response studies in toxicology is to determine an \u2018alert\u2019 concentration where a critical level of the response variable is exceeded. In a classical observation-based approach, only measured concentrations are considered as potential alert concentrations. Alternatively, a parametric curve is fitted to the data that describes the relationship between concentration and response. For a prespecified effect level, both an absolute estimate of the alert concentration and an estimate of the lowest concentration where the effect level is exceeded significantly are of interest.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In a simulation study for gene expression data, we compared the observation-based and the model-based approach for both absolute and significant exceedance of the prespecified effect level. Results show that, compared to the observation-based approach, the model-based approach overestimates the true alert concentration less often and more frequently leads to a valid estimate, especially for genes with large variance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>The code used for the simulation studies is available via the GitHub repository: https:\/\/github.com\/FKappenberg\/Paper-IdentificationAlertConcentrations.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab043","type":"journal-article","created":{"date-parts":[[2021,1,25]],"date-time":"2021-01-25T16:04:03Z","timestamp":1611590643000},"page":"1990-1996","source":"Crossref","is-referenced-by-count":10,"title":["Comparison of observation-based and model-based identification of alert concentrations from concentration\u2013expression data"],"prefix":"10.1093","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8066-5333","authenticated-orcid":false,"given":"Franziska","family":"Kappenberg","sequence":"first","affiliation":[{"name":"Department of Statistics, TU Dortmund University , Dortmund 44221, Germany"}]},{"given":"Marianna","family":"Grinberg","sequence":"additional","affiliation":[{"name":"Department of Statistics, TU Dortmund University , Dortmund 44221, Germany"}]},{"given":"Xiaoqi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Division of Biostatistics, German Cancer Research Center (DKFZ) , Heidelberg 69120, Germany"}]},{"given":"Annette","family":"Kopp-Schneider","sequence":"additional","affiliation":[{"name":"Division of Biostatistics, German Cancer Research Center (DKFZ) , Heidelberg 69120, Germany"}]},{"given":"Jan G","family":"Hengstler","sequence":"additional","affiliation":[{"name":"Department of Toxikologie\/Systemtoxikologie, Leibniz Research Centre for Working Environment and Human Factors (IfADo), TU Dortmund University , Dortmund 44139, Germany"}]},{"given":"J\u00f6rg","family":"Rahnenf\u00fchrer","sequence":"additional","affiliation":[{"name":"Department of Statistics, TU Dortmund University , Dortmund 44221, Germany"}]}],"member":"286","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"2023061310303082900_btab043-B1","doi-asserted-by":"crossref","first-page":"2635","DOI":"10.1007\/s00204-019-02520-8","article-title":"Model-based estimation of lowest observed effect concentration from replicate experiments to identify potential biomarkers of in vitro neurotoxicity","volume":"93","author":"Calderazzo","year":"2019","journal-title":"Arch. 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