{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T09:20:06Z","timestamp":1774689606700,"version":"3.50.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"18","license":[{"start":{"date-parts":[[2021,3,3]],"date-time":"2021-03-03T00:00:00Z","timestamp":1614729600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"publisher","award":["NNF10CC1016517"],"award-info":[{"award-number":["NNF10CC1016517"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Foundation","doi-asserted-by":"publisher","award":["NNF14OC0009473"],"award-info":[{"award-number":["NNF14OC0009473"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Australian Research Council Centre of Excellence in Synthetic Biology","award":["CE200100029"],"award-info":[{"award-number":["CE200100029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>We achieve a significant improvement in thermodynamic-based flux analysis (TFA) by introducing multivariate treatment of thermodynamic variables and leveraging component contribution, the state-of-the-art implementation of the group contribution methodology. Overall, the method greatly reduces the uncertainty of thermodynamic variables.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We present multiTFA, a Python implementation of our framework. We evaluated our application using the core Escherichia coli model and achieved a median reduction of 6.8\u2009kJ\/mol in reaction Gibbs free energy ranges, while three out of 12 reactions in glycolysis changed from reversible to irreversible.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>Our framework along with documentation is available on https:\/\/github.com\/biosustain\/multitfa.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab151","type":"journal-article","created":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T15:15:21Z","timestamp":1614611721000},"page":"3064-3066","source":"Crossref","is-referenced-by-count":13,"title":["multiTFA: a Python package for multi-variate thermodynamics-based flux analysis"],"prefix":"10.1093","volume":"37","author":[{"given":"Vishnuvardhan","family":"Mahamkali","sequence":"first","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD 4072, Australia"}]},{"given":"Tim","family":"McCubbin","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD 4072, Australia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2406-1978","authenticated-orcid":false,"given":"Moritz Emanuel","family":"Beber","sequence":"additional","affiliation":[{"name":"The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark , 2800 Kgs. Lyngby, Denmark"}]},{"given":"Elad","family":"Noor","sequence":"additional","affiliation":[{"name":"Department of Plant and Environmental Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel"}]},{"given":"Esteban","family":"Marcellin","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD 4072, Australia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8191-3511","authenticated-orcid":false,"given":"Lars Keld","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland , Brisbane, QLD 4072, Australia"},{"name":"The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark , 2800 Kgs. 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