{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:47:21Z","timestamp":1773967641897,"version":"3.50.1"},"reference-count":3,"publisher":"Oxford University Press (OUP)","issue":"19","license":[{"start":{"date-parts":[[2021,3,23]],"date-time":"2021-03-23T00:00:00Z","timestamp":1616457600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001823","name":"Ministry of Education, Youths, and Sports","doi-asserted-by":"crossref","award":["MSMT-15739\/2019-8"],"award-info":[{"award-number":["MSMT-15739\/2019-8"]}],"id":[{"id":"10.13039\/501100001823","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000921","name":"European Cooperation in Science and Technology","doi-asserted-by":"publisher","award":["CA16107"],"award-info":[{"award-number":["CA16107"]}],"id":[{"id":"10.13039\/501100000921","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AFRI Education and Workforce Development Postdoctoral Fellowship","award":["2018-08122"],"award-info":[{"award-number":["2018-08122"]}]},{"DOI":"10.13039\/100005825","name":"U.S. Department of Agriculture, National Institute of Food and Agriculture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Searching for amino acid or nucleic acid sequences unique to one organism may be challenging depending on size of the available datasets. K-mer elimination by cross-reference (KEC) allows users to quickly and easily find unique sequences by providing target and non-target sequences. Due to its speed, it can be used for datasets of genomic size and can be run on desktop or laptop computers with modest specifications.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>KEC is freely available for non-commercial purposes. Source code and executable binary files compiled for Linux, Mac and Windows can be downloaded from https:\/\/github.com\/berybox\/KEC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab196","type":"journal-article","created":{"date-parts":[[2021,3,22]],"date-time":"2021-03-22T20:14:27Z","timestamp":1616444067000},"page":"3349-3350","source":"Crossref","is-referenced-by-count":10,"title":["KEC: unique sequence search by K-mer exclusion"],"prefix":"10.1093","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2680-3958","authenticated-orcid":false,"given":"Pavel","family":"Beran","sequence":"first","affiliation":[{"name":"Department of Genetics and Agricultural Biotechnology, Biotechnological Centre, University of South Bohemia, Faculty of Agriculture , 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"given":"Dagmar","family":"Stehl\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agricultural Biotechnology, Biotechnological Centre, University of South Bohemia, Faculty of Agriculture , 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]},{"given":"Stephen P","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, The Ohio State University , Columbus, OH 43210, USA"}]},{"given":"Vladislav","family":"\u010curn","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agricultural Biotechnology, Biotechnological Centre, University of South Bohemia, Faculty of Agriculture , 37005 \u010cesk\u00e9 Bud\u011bjovice, Czech Republic"}]}],"member":"286","published-online":{"date-parts":[[2021,3,23]]},"reference":[{"key":"2023051608261159600_btab196-B1","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1094\/PDIS-10-18-1819-RE","article-title":"Development of the automated primer design workflow uniqprimer and diagnostic primers for the broad-host-range plant pathogen Dickeya dianthicola","volume":"103","author":"Karim","year":"2019","journal-title":"Plant Dis"},{"key":"2023051608261159600_btab196-B2","doi-asserted-by":"crossref","first-page":"14298","DOI":"10.1038\/s41598-018-32295-4","article-title":"Development of a genome-informed loop-mediated isothermal amplification assay for rapid and specific detection of Xanthomonas euvesicatoria","volume":"8","author":"Larrea-Sarmiento","year":"2018","journal-title":"Sci. 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Bioinf"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bioinformatics\/advance-article-pdf\/doi\/10.1093\/bioinformatics\/btab196\/36940227\/btab196.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/19\/3349\/50338428\/btab196.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/19\/3349\/50338428\/btab196.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T08:38:40Z","timestamp":1684226320000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/37\/19\/3349\/6182676"}},"subtitle":[],"editor":[{"given":"Peter","family":"Robinson","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2021,3,23]]},"references-count":3,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2021,10,11]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btab196","relation":{},"ISSN":["1367-4803","1367-4811"],"issn-type":[{"value":"1367-4803","type":"print"},{"value":"1367-4811","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,10,1]]},"published":{"date-parts":[[2021,3,23]]}}}