{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T03:08:15Z","timestamp":1763867295738,"version":"3.37.3"},"reference-count":8,"publisher":"Oxford University Press (OUP)","issue":"22","license":[{"start":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:00:00Z","timestamp":1621209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Scientific and Technological Research Council of Turkey \u2013 Chemistry and Biology Research Support Group","award":["118Z957"],"award-info":[{"award-number":["118Z957"]}]},{"name":"Middle East Technical University \u2013 Scientific Research Projects","award":["108-2021-10640"],"award-info":[{"award-number":["108-2021-10640"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Next generation sequencing enabled the fast accumulation of genomic data at public repositories. This technology also made it possible to better understand the regulation of gene expression by transcription factors (TFs) and various chromatin-associated proteins through the integration of chromatin immunoprecipitation (ChIP-Seq). The Cistrome Project has become one of the indispensable research portals for biologists to access and analyze data generated with thousands of ChIP-Seq experiments. Integrative motif analysis on shared binding regions among a set of experiments is not yet achievable despite a set of search and analysis tools provided by Cistrome via its web interface and the Galaxy framework.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>We implemented a python command-line tool for searching binding sequences of a TF common to multiple ChIP-Seq experiments. We use the peaks in the Cistrome database as identified by MACS 2.0 for each experiment and identify shared peak regions in a genomic locus of interest. We then scan these regions for binding sequences using a binding motif of a TF obtained from the JASPAR database. MotifGenie is developed in collaboration with molecular biologists and its findings are corroborated by laboratory experiments.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>MotifGenie is freely available at https:\/\/github.com\/ceragoguztuzun\/MotifGenie.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab379","type":"journal-article","created":{"date-parts":[[2021,5,13]],"date-time":"2021-05-13T19:22:08Z","timestamp":1620933728000},"page":"4238-4239","source":"Crossref","is-referenced-by-count":3,"title":["MotifGenie: a Python application for searching transcription factor binding sequences using ChIP-Seq datasets"],"prefix":"10.1093","volume":"37","author":[{"given":"Cerag","family":"Oguztuzun","sequence":"first","affiliation":[{"name":"Department of Computer Engineering, Bilkent University , Ankara 06800, Turkey"}]},{"given":"Pelin","family":"Yasar","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Middle East Technical University , Ankara 06800, Turkey"}]},{"given":"Kerim","family":"Yavuz","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Middle East Technical University , Ankara 06800, Turkey"}]},{"given":"Mesut","family":"Muyan","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Middle East Technical University , Ankara 06800, Turkey"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5532-0154","authenticated-orcid":false,"given":"Tolga","family":"Can","sequence":"additional","affiliation":[{"name":"Department of Computer Engineering, Middle East Technical University , Ankara 06800, Turkey"}]}],"member":"286","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"2023051607084313200_btab379-B1","doi-asserted-by":"crossref","first-page":"W3","DOI":"10.1093\/nar\/gkw343","article-title":"The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2016 update","volume":"44","author":"Afgan","year":"2016","journal-title":"Nucleic Acids Res"},{"key":"2023051607084313200_btab379-B2","doi-asserted-by":"crossref","first-page":"e1000852","DOI":"10.1371\/journal.pcbi.1000852","article-title":"The construction and use of log-odds substitution scores for multiple sequence alignment","volume":"6","author":"Altschul","year":"2010","journal-title":"PLoS Comput. Biol"},{"key":"2023051607084313200_btab379-B3","first-page":"D87","article-title":"JASPAR 2020: update of the open-access database of transcription factor binding profiles","volume":"48","author":"Fornes","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"2023051607084313200_btab379-B4","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1101\/gr.229102","article-title":"The human genome browser at UCSC","volume":"12","author":"Kent","year":"2002","journal-title":"Genome Res"},{"key":"2023051607084313200_btab379-B6","doi-asserted-by":"crossref","first-page":"R83","DOI":"10.1186\/gb-2011-12-8-r83","article-title":"Cistrome: an integrative platform for transcriptional regulation studies","volume":"12","author":"Liu","year":"2011","journal-title":"Genome Biol"},{"key":"2023051607084313200_btab379-B7","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.celrep.2018.03.056","article-title":"Transcriptional dysregulation of MYC reveals common enhancer-docking mechanism","volume":"23","author":"Schuijers","year":"2018","journal-title":"Cell Rep"},{"key":"2023051607084313200_btab379-B8","article-title":"A CpG island promoter drives the CXXC5 gene expression","author":"Yasar","year":"2021","journal-title":"submitted"},{"key":"2023051607084313200_btab379-B9","doi-asserted-by":"crossref","first-page":"R137","DOI":"10.1186\/gb-2008-9-9-r137","article-title":"Model-based analysis of ChIP-Seq (MACS)","volume":"9","author":"Zhang","year":"2008","journal-title":"Genome Biol"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/bioinformatics\/advance-article-pdf\/doi\/10.1093\/bioinformatics\/btab379\/38576428\/btab379.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/22\/4238\/50335097\/btab379.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/37\/22\/4238\/50335097\/btab379.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,16]],"date-time":"2023-05-16T07:09:59Z","timestamp":1684220999000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/37\/22\/4238\/6276986"}},"subtitle":[],"editor":[{"given":"Peter","family":"Robinson","sequence":"additional","affiliation":[]}],"short-title":[],"issued":{"date-parts":[[2021,5,17]]},"references-count":8,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2021,11,18]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btab379","relation":{},"ISSN":["1367-4803","1367-4811"],"issn-type":[{"type":"print","value":"1367-4803"},{"type":"electronic","value":"1367-4811"}],"subject":[],"published-other":{"date-parts":[[2021,11,15]]},"published":{"date-parts":[[2021,5,17]]}}}