{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:49:16Z","timestamp":1773809356091,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"22","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"AFM Telethon"},{"name":"Marion Elisabeth Brancher"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Cardiomyocytes derived from stem cells are closely followed, notably since the discovery in 2007 of human induced pluripotent stem cells (hiPSC). Cardiomyocytes (hiPSC-CM) derived from hiPSC are indeed more and more used to study specific cardiac diseases as well as for developing novel applications such as drug safety experiments. Robust dedicated tools to characterize hiPSC-CM are now required. The hiPSC-CM morphology constitutes an important parameter since these cells do not demonstrate the expected rod shape, characteristic of native human cardiomyocytes. Similarly, the presence, the density and the organization of contractile structures would be a valuable parameter to study. Precise measurements of such characteristics would be useful in many situations: for describing pathological conditions, for pharmacological screens or even for studies focused on the hiPSC-CM maturation process.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>For this purpose, we developed a MATLAB based image analysis toolbox, which gives accurate values for cellular morphology parameters as well as for the contractile cell organization.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>To demonstrate the power of this automated image analysis, we used a commercial maturation medium intended to promote the maturation status of hiPSC-CM, and compare the parameters with the ones obtained with standard culture medium, and with freshly dissociated mouse cardiomyocytes.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab400","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T21:58:13Z","timestamp":1622152693000},"page":"4209-4215","source":"Crossref","is-referenced-by-count":8,"title":["MorphoScript: a dedicated analysis to assess the morphology and contractile structures of cardiomyocytes derived from stem cells"],"prefix":"10.1093","volume":"37","author":[{"given":"Tess","family":"Homan","sequence":"first","affiliation":[{"name":"Univ Lyon, Univ Claude Bernard Lyon 1, CNRS, Institut Lumi\u00e8re Mati\u00e8re , Villeurbanne F-69622, France"}]},{"given":"H\u00e9l\u00e8ne","family":"Delano\u00eb-Ayari","sequence":"additional","affiliation":[{"name":"Univ Lyon, Univ Claude Bernard Lyon 1, CNRS, Institut Lumi\u00e8re Mati\u00e8re , Villeurbanne F-69622, France"}]},{"given":"Albano C","family":"Meli","sequence":"additional","affiliation":[{"name":"PhyMedExp, INSERM, CNRS, University of Montpellier , Montpellier 34 090, France"}]},{"given":"Olivier","family":"Cazorla","sequence":"additional","affiliation":[{"name":"PhyMedExp, INSERM, CNRS, University of Montpellier , Montpellier 34 090, France"}]},{"given":"Csilla","family":"Gergely","sequence":"additional","affiliation":[{"name":"L2C, University of Montpellier, CNRS , Montpellier 34 090, France"}]},{"given":"Alexandre","family":"Mejat","sequence":"additional","affiliation":[{"name":"Claude Bernard University, Lyon 1, Universit\u00e9 de Lyon , Lyon F-69622, France"},{"name":"Neuromyogene Institut , Lyon 69008, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8185-2229","authenticated-orcid":false,"given":"Philippe","family":"Chevalier","sequence":"additional","affiliation":[{"name":"Claude Bernard University, Lyon 1, Universit\u00e9 de Lyon , Lyon F-69622, France"},{"name":"Neuromyogene Institut , Lyon 69008, France"},{"name":"Hospices Civils de Lyon, Service de Rythmologie , Bron 69677, France"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3100-0807","authenticated-orcid":false,"given":"Adrien","family":"Moreau","sequence":"additional","affiliation":[{"name":"PhyMedExp, INSERM, CNRS, University of Montpellier , Montpellier 34 090, France"}]}],"member":"286","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"2023051607110670800_btab400-B1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.3390\/jcm7110423","article-title":"Post-translational modifications and diastolic calcium leak associated to the novel RyR2-D3638A mutation lead to CPVT in patient-specific hiPSC-derived cardiomyocytes","volume":"7","author":"Acimovic","year":"2018","journal-title":"J. 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