{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T09:33:57Z","timestamp":1780392837026,"version":"3.54.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T00:00:00Z","timestamp":1639526400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Youth Science and Technology Talent Support Project of Jilin Province","award":["QT202109"],"award-info":[{"award-number":["QT202109"]}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M651204"],"award-info":[{"award-number":["2019M651204"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Viruses, the most abundant biological entities on earth, are important components of microbial communities, and as major human pathogens, they are responsible for human mortality and morbidity. The identification of viral sequences from metagenomes is critical for viral analysis. As massive quantities of short sequences are generated by next-generation sequencing, most methods utilize discrete and sparse one-hot vectors to encode nucleotide sequences, which are usually ineffective in viral identification.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In this article, Virtifier, a deep learning-based viral identifier for sequences from metagenomic data is proposed. It includes a meaningful nucleotide sequence encoding method named Seq2Vec and a variant viral sequence predictor with an attention-based long short-term memory (LSTM) network. By utilizing a fully trained embedding matrix to encode codons, Seq2Vec can efficiently extract the relationships among those codons in a nucleotide sequence. Combined with an attention layer, the LSTM neural network can further analyze the codon relationships and sift the parts that contribute to the final features. Experimental results of three datasets have shown that Virtifier can accurately identify short viral sequences (&amp;lt;500\u2009bp) from metagenomes, surpassing three widely used methods, VirFinder, DeepVirFinder and PPR-Meta. Meanwhile, a comparable performance was achieved by Virtifier at longer lengths (&amp;gt;5000\u2009bp).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>A Python implementation of Virtifier and the Python code developed for this study have been provided on Github https:\/\/github.com\/crazyinter\/Seq2Vec. The RefSeq genomes in this article are available in VirFinder at https:\/\/dx.doi.org\/10.1186\/s40168-017-0283-5. The CAMI Challenge Dataset 3 CAMI_high dataset in this article is available in CAMI at https:\/\/data.cami-challenge.org\/participate. The real human gut metagenomes in this article are available at https:\/\/dx.doi.org\/10.1101\/gr.142315.112.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btab845","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T12:11:09Z","timestamp":1639397469000},"page":"1216-1222","source":"Crossref","is-referenced-by-count":47,"title":["Virtifier: a deep learning-based identifier for viral sequences from metagenomes"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7719-426X","authenticated-orcid":false,"given":"Yan","family":"Miao","sequence":"first","affiliation":[{"name":"College of Communication Engineering, Jilin University , Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Communication Engineering, Jilin University , Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Communication Engineering, Jilin University , Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0883-2453","authenticated-orcid":false,"given":"Yun","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Communication Engineering, Jilin University , Changchun 130022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,15]]},"reference":[{"key":"2023020108573212500_btab845-B1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. 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