{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,27]],"date-time":"2025-12-27T21:03:58Z","timestamp":1766869438900,"version":"3.37.3"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T00:00:00Z","timestamp":1649203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Research Foundation\u2014Flanders","award":["1S64720N"],"award-info":[{"award-number":["1S64720N"]}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["PDMt2\/21\/038"],"award-info":[{"award-number":["PDMt2\/21\/038"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Missing regions in short-read assemblies of prokaryote genomes are often attributed to biases in sequencing technologies and to repetitive elements, the former resulting in low sequencing coverage of certain loci and the latter to unresolved loops in the de novo assembly graph. We developed SASpector, a command-line tool that compares short-read assemblies (draft genomes) to their corresponding closed assemblies and extracts missing regions to analyze them at the sequence and functional level. SASpector allows to benchmark the need for resolved genomes, can be integrated into pipelines to control the quality of assemblies, and could be used for comparative investigations of missingness in assemblies for which both short-read and long-read data are available in the public databases.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>SASpector is available at https:\/\/github.com\/LoGT-KULeuven\/SASpector. The tool is implemented in Python3 and available through pip and Docker (0mician\/saspector).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac208","type":"journal-article","created":{"date-parts":[[2022,4,6]],"date-time":"2022-04-06T13:43:51Z","timestamp":1649252631000},"page":"2920-2921","source":"Crossref","is-referenced-by-count":1,"title":["SASpector: analysis of missing genomic regions in draft genomes of prokaryotes"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7826-3378","authenticated-orcid":false,"given":"C\u00e9dric","family":"Lood","sequence":"first","affiliation":[{"name":"Department of Microbial and Molecular Systems, KU Leuven , 3001 Leuven, Belgium"},{"name":"Department of Biosystems, KU Leuven , 3001 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Correa Rojo","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, KU Leuven , 3001 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deniz","family":"Sinar","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, KU Leuven , 3001 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Verkinderen","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, KU Leuven , 3001 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob","family":"Lavigne","sequence":"additional","affiliation":[{"name":"Department of Biosystems, KU Leuven , 3001 Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera van","family":"Noort","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Systems, KU Leuven , 3001 Leuven, Belgium"},{"name":"Institute of Biology, Leiden University , 2333 Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,4,6]]},"reference":[{"key":"2023020109145938200_btac208-B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/jpb.1000420","article-title":"Computational errors and biases in short read next generation sequencing","volume":"10","author":"Abnizova","year":"2017","journal-title":"J. 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