{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T02:22:27Z","timestamp":1781749347106,"version":"3.54.5"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2022,4,21]],"date-time":"2022-04-21T00:00:00Z","timestamp":1650499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Carver Charitable Trust and Iowa State University Foundation"},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"crossref","award":["PID2019-105017RB-I00"],"award-info":[{"award-number":["PID2019-105017RB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ICREA, ICREA-Academia 2019"},{"DOI":"10.13039\/100016077","name":"EU","doi-asserted-by":"publisher","award":["PhasAge\/H2020-WIDESPREAD-2020-5"],"award-info":[{"award-number":["PhasAge\/H2020-WIDESPREAD-2020-5"]}],"id":[{"id":"10.13039\/100016077","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Spanish Ministry of Science and Innovation with a Juan de la Cierva Incorporacion","award":["IJC2019-041039-I"],"award-info":[{"award-number":["IJC2019-041039-I"]}]},{"DOI":"10.13039\/501100004442","name":"National Science Centre, Poland","doi-asserted-by":"publisher","award":["2020\/39\/B\/NZ2\/01301"],"award-info":[{"award-number":["2020\/39\/B\/NZ2\/01301"]}],"id":[{"id":"10.13039\/501100004442","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>Protein aggregation is associated with many human disorders and constitutes a major bottleneck for producing therapeutic proteins. Our knowledge of the human protein structures repertoire has dramatically increased with the recent development of the AlphaFold (AF) deep-learning method. This structural information can be used to understand better protein aggregation properties and the rational design of protein solubility. This article uses the Aggrescan3D (A3D) tool to compute the structure-based aggregation predictions for the human proteome and make the predictions available in a database form. In the A3D database, we analyze the AF-predicted human protein structures (for over 20.5 thousand unique Uniprot IDs) in terms of their aggregation properties using the A3D tool. Each entry of the A3D database provides a detailed analysis of the structure-based aggregation propensity computed with A3D. The A3D database implements simple but useful graphical tools for visualizing and interpreting protein structure datasets. It also enables testing the influence of user-selected mutations on protein solubility and stability, all integrated into a user-friendly interface.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>A3D database is freely available at: http:\/\/biocomp.chem.uw.edu.pl\/A3D2\/hproteome. The data underlying this article are available in the article and in its online supplementary material.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Supplementary information<\/jats:title>\n                    <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac215","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T07:33:41Z","timestamp":1650353621000},"page":"3121-3123","source":"Crossref","is-referenced-by-count":13,"title":["A3D database: structure-based predictions of protein aggregation for the human proteome"],"prefix":"10.1093","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1832-4364","authenticated-orcid":false,"given":"Aleksandra E","family":"Badaczewska-Dawid","sequence":"first","affiliation":[{"name":"Department of Chemistry, Iowa State University , Ames, 50011 IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9179-6371","authenticated-orcid":false,"given":"Javier","family":"Garcia-Pardo","sequence":"additional","affiliation":[{"name":"Institut de Biotecnologia i de Biomedicina (IBB) and Departament de Bioqu\u00edmica i Biologia Molecular, Universitat Aut\u00f2noma de Barcelona , 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksander","family":"Kuriata","sequence":"additional","affiliation":[{"name":"Biological and Chemical Research Center, Faculty of Chemistry, University of Warsaw , Warsaw 02-093, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Pujols","sequence":"additional","affiliation":[{"name":"Institut de Biotecnologia i de Biomedicina (IBB) and Departament de Bioqu\u00edmica i Biologia Molecular, Universitat Aut\u00f2noma de Barcelona , 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Ventura","sequence":"additional","affiliation":[{"name":"Institut de Biotecnologia i de Biomedicina (IBB) and Departament de Bioqu\u00edmica i Biologia Molecular, Universitat Aut\u00f2noma de Barcelona , 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7623-0935","authenticated-orcid":false,"given":"Sebastian","family":"Kmiecik","sequence":"additional","affiliation":[{"name":"Biological and Chemical Research Center, Faculty of Chemistry, University of Warsaw , Warsaw 02-093, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,21]]},"reference":[{"key":"2023041402570676100_","doi-asserted-by":"crossref","first-page":"856","DOI":"10.3390\/cells8080856","article-title":"Computational assessment of bacterial protein structures indicates a selection against aggregation","volume":"8","author":"Carija","year":"2019","journal-title":"Cells"},{"key":"2023041402570676100_","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.1038\/s41598-020-80746-8","article-title":"Generation and functional characterization of a single-chain variable fragment (scFv) of the anti-FGF2 3F12E7 monoclonal antibody","volume":"11","author":"de Aguiar","year":"2021","journal-title":"Sci. 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