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We proposed CeCaFLUX, the first user-friendly web server that derives metabolic flux distribution from instationary 13C-labeled data. Flux optimization and statistical analysis are achieved through an evolutionary optimization in a parallel manner. It can visualize the flux optimizing process in real-time and the ultimate flux outcome. It will also function as a database to enhance the consistency and to facilitate sharing of flux studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Availability and implementation<\/jats:title><jats:p>CeCaFLUX is freely available at https:\/\/www.cecaflux.net, the source code can be downloaded at https:\/\/github.com\/zhzhd82\/CeCaFLUX.<\/jats:p><\/jats:sec><jats:sec><jats:title>Supplementary information<\/jats:title><jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p><\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac341","type":"journal-article","created":{"date-parts":[[2022,5,20]],"date-time":"2022-05-20T23:33:46Z","timestamp":1653089626000},"page":"3481-3483","source":"Crossref","is-referenced-by-count":0,"title":["CeCaFLUX: the first web server for standardized and visual instationary 13C metabolic flux analysis"],"prefix":"10.1093","volume":"38","author":[{"given":"Zhentao","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Information and Computing Science Guizhou Province, Guizhou Normal University , Guiyang, Guizhou, China"},{"name":"College of Computer Science and Technology, Guizhou University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengdong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Information and Computing Science Guizhou Province, Guizhou Normal University , Guiyang, Guizhou, China"},{"name":"College of Mathematics and Information Science, Guiyang University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Mathematics and Information Science, Guiyang University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Mathematical Science, Guizhou Normal University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyao","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Information and Computing Science Guizhou Province, Guizhou Normal University , Guiyang, Guizhou, China"},{"name":"College of Computer Science and Technology, Guizhou University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7313-3494","authenticated-orcid":false,"given":"Tie","family":"Shen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Information and Computing Science Guizhou Province, Guizhou Normal University , Guiyang, Guizhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,5,20]]},"reference":[{"key":"2023041407595983300_","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/978-1-4939-7295-1_21","article-title":"Two-scale (13)C metabolic flux analysis for metabolic engineering","volume":"1671","author":"Ando","year":"2018","journal-title":"Methods Mol. 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