{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:34:13Z","timestamp":1772138053444,"version":"3.50.1"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T00:00:00Z","timestamp":1653350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Wellcome Trust and Royal Society","award":["204290\/Z\/16\/Z"],"award-info":[{"award-number":["204290\/Z\/16\/Z"]}]},{"DOI":"10.13039\/501100000268","name":"BBSRC","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Multiple Sclerosis Society Tissue Bank"},{"DOI":"10.13039\/501100000381","name":"Multiple Sclerosis Society of Great Britain and Northern Ireland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000381","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>With continually improved instrumentation, Fourier transform infrared (FTIR) microspectroscopy can now be used to capture thousands of high-resolution spectra for chemical characterization of a sample. The spatially resolved nature of this method lends itself well to histological profiling of complex biological specimens. However, current software can make joint analysis of multiple samples challenging and, for large datasets, computationally infeasible.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>To overcome these limitations, we have developed Photizo\u2014an open-source Python library enabling high-throughput spectral data pre-processing, visualization and downstream analysis, including principal component analysis, clustering, macromolecular quantification and mapping. Photizo can be used for analysis of data without a spatial component, as well as spatially resolved data, obtained e.g. by scanning mode IR microspectroscopy and IR imaging by focal plane array detector.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>The code underlying this article is available at https:\/\/github.com\/DendrouLab\/Photizo with access to example data available at https:\/\/zenodo.org\/record\/6417982#.Yk2O9TfMI6A.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac346","type":"journal-article","created":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T08:27:54Z","timestamp":1653380874000},"page":"3490-3492","source":"Crossref","is-referenced-by-count":9,"title":["Photizo: an open-source library for cross-sample analysis of FTIR spectroscopy data"],"prefix":"10.1093","volume":"38","author":[{"given":"Melissa","family":"Grant-Peters","sequence":"first","affiliation":[{"name":"Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford , Oxford OX3 7BN, UK"}]},{"given":"Charlotte","family":"Rich-Griffin","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford , Oxford OX3 7BN, UK"}]},{"given":"Jonathan E","family":"Grant-Peters","sequence":"additional","affiliation":[{"name":"Mathematical Institute, University of Oxford , Oxford OX2 6GG, UK"}]},{"given":"Gianfelice","family":"Cinque","sequence":"additional","affiliation":[{"name":"Diamond Light Source , Didcot OX11 0DE, UK"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1179-4021","authenticated-orcid":false,"given":"Calliope A","family":"Dendrou","sequence":"additional","affiliation":[{"name":"Nuffield Department of Medicine, Wellcome Centre for Human Genetics, University of Oxford , Oxford OX3 7BN, UK"}]}],"member":"286","published-online":{"date-parts":[[2022,5,24]]},"reference":[{"key":"2023041408094954500_","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1038\/nprot.2014.110","article-title":"Using Fourier transform IR spectroscopy to analyze biological materials","volume":"9","author":"Baker","year":"2014","journal-title":"Nat. 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