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It is widely accepted that automation is critical to the production of neuronal morphology. Despite previous survey papers about neuron tracing from light microscopy data in the last decade, thanks to the rapid development of the field, there is a need to update recent progress in a review focusing on new methods and remarkable applications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This review outlines neuron tracing in various scenarios with the goal to help the community understand and navigate tools and resources. We describe the status, examples and accessibility of automatic neuron tracing. We survey recent advances of the increasingly popular deep-learning enhanced methods. We highlight the semi-automatic methods for single neuron tracing of mammalian whole brains as well as the resulting datasets, each containing thousands of full neuron morphologies. Finally, we exemplify the commonly used datasets and metrics for neuron tracing bench testing.<\/jats:p><\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac712","type":"journal-article","created":{"date-parts":[[2022,10,28]],"date-time":"2022-10-28T05:01:31Z","timestamp":1666933291000},"page":"5329-5339","source":"Crossref","is-referenced-by-count":53,"title":["Neuron tracing from light microscopy images: automation, deep learning and bench testing"],"prefix":"10.1093","volume":"38","author":[{"given":"Yufeng","family":"Liu","sequence":"first","affiliation":[{"name":"School of Biological Science and Medical Engineering, Southeast University , Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Computer Science and Engineering, Southeast University , Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgio A","family":"Ascoli","sequence":"additional","affiliation":[{"name":"Center for Neural Informatics, Structures, & Plasticity, Krasnow Institute for Advanced Study, George Mason University , Fairfax, VA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangning","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Brain Science, The First Affiliated Hospital of Anhui Medical University , Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9548-6183","authenticated-orcid":false,"given":"Lijuan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological Science and Medical Engineering, Southeast University , Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,10,27]]},"reference":[{"key":"2022121418411248300_btac712-B1","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s12021-016-9310-0","article-title":"Automated neuron tracing methods: an updated account","volume":"14","author":"Acciai","year":"2016","journal-title":"Neuroinformatics"},{"key":"2022121418411248300_btac712-B2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1109\/TITB.2002.1006304","article-title":"Rapid automated three-dimensional tracing of neurons from confocal image stacks","volume":"6","author":"Al-Kofahi","year":"2002","journal-title":"IEEE Trans. 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