{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T10:14:20Z","timestamp":1779876860770,"version":"3.53.1"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T00:00:00Z","timestamp":1669766400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006095","name":"South-Eastern Norway Regional Health Authority","doi-asserted-by":"publisher","award":["2019074"],"award-info":[{"award-number":["2019074"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006095","name":"South-Eastern Norway Regional Health Authority","doi-asserted-by":"publisher","award":["2019030"],"award-info":[{"award-number":["2019030"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Motivation<\/jats:title>\n                  <jats:p>Droplet digital PCR (ddPCR) holds great promises for investigating DNA methylation with high sensitivity. Yet, the lack of methods for analyzing ddPCR DNA methylation data has resulted in users processing the data manually at the expense of standardization.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>PoDCall is an R package performing automated calling of positive droplets, quantification and normalization of methylation levels in ddPCR experiments. A Shiny application provides users with an intuitive and interactive interface to access PoDCall functionalities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>The PoDCall R package is freely available on Bioconductor at https:\/\/bioconductor.org\/packages\/PoDCall\/. The Shiny application can be executed from the R console using the wrapper function PoDCall::podcallShiny().<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Supplementary information<\/jats:title>\n                  <jats:p>Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btac766","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T22:31:28Z","timestamp":1669761088000},"source":"Crossref","is-referenced-by-count":10,"title":["PoDCall: positive droplet calling and normalization of droplet digital PCR DNA methylation data"],"prefix":"10.1093","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6239-4357","authenticated-orcid":false,"given":"Marine","family":"Jeanmougin","sequence":"first","affiliation":[{"name":"Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital , Oslo 0379, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Petter","family":"Brodal","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital , Oslo 0379, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Dietrichson Pharo","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital , Oslo 0379, Norway"},{"name":"Department of Biosciences, The Faculty of Mathematics and Natural Sciences, University of Oslo , Oslo 0371, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hege Marie","family":"Vedeld","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital , Oslo 0379, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guro Elisabeth","family":"Lind","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Institute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital , Oslo 0379, Norway"},{"name":"Department of Biosciences, The Faculty of Mathematics and Natural Sciences, University of Oslo , Oslo 0371, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,11,30]]},"reference":[{"key":"2023010805370692400_btac766-B1","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.12688\/f1000research.9022.1","article-title":"ddpcr: an R package and web application for analysis of droplet digital PCR data","volume":"5","author":"Attali","year":"2016","journal-title":"F1000Research"},{"key":"2023010805370692400_btac766-B2","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1038\/ng.2334","article-title":"Structural haplotypes and recent evolution of the human 17q21.31 region","volume":"44","author":"Boettger","year":"2012","journal-title":"Nat. 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