{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:34:31Z","timestamp":1772138071080,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"11","funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>Natural killer (NK) cells are essential components of the innate immune system, with their activity significantly regulated by Killer cell Immunoglobulin-like Receptors (KIRs). The diversity and structural complexity of KIR genes present significant challenges for accurate genotyping, essential for understanding NK cell functions and their implications in health and disease. Traditional genotyping methods struggle with the variable nature of KIR genes, leading to inaccuracies that can impede immunogenetic research. These challenges extend to high-quality phased assemblies, which have been recently popularized by the Human Pangenome Consortium. This article introduces BAKIR (Biologically informed Annotator for KIR locus), a tailored computational tool designed to overcome the challenges of KIR genotyping and annotation on high-quality, phased genome assemblies. BAKIR aims to enhance the accuracy of KIR gene annotations by structuring its annotation pipeline around identifying key functional mutations, thereby improving the identification and subsequent relevance of gene and allele calls. It uses a multi-stage mapping, alignment, and variant calling process to ensure high-precision gene and allele identification, while also maintaining high recall for sequences that are significantly mutated or truncated relative to the known allele database. BAKIR has been evaluated on a subset of the HPRC assemblies, where BAKIR was able to improve many of the associated annotations and call novel variants. BAKIR is freely available on GitHub, offering ease of access and use through multiple installation methods, including pip, conda, and singularity container, and is equipped with a user-friendly command-line interface, thereby promoting its adoption in the scientific community.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>BAKIR is available at github.com\/algo-cancer\/bakir<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btae622","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T23:50:39Z","timestamp":1729122639000},"source":"Crossref","is-referenced-by-count":2,"title":["Biologically-informed killer cell immunoglobulin-like receptor gene annotation tool"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9451-3541","authenticated-orcid":false,"given":"Michael K B","family":"Ford","sequence":"first","affiliation":[{"name":"National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD, 20892,","place":["United States"]}]},{"given":"Ananth","family":"Hari","sequence":"additional","affiliation":[{"name":"National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD, 20892,","place":["United States"]},{"name":"Department of Electrical Engineering, University of Maryland , 2410 A.V. Williams Building , College Park, MD, 20742,","place":["United States"]}]},{"given":"Qinghui","family":"Zhou","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Computer Science, University of Victoria , 3800 Finnerty Rd , Victoria, BC, V8P 5C2,","place":["Canada"]}]},{"given":"Ibrahim","family":"Numanagi\u0107","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Computer Science, University of Victoria , 3800 Finnerty Rd , Victoria, BC, V8P 5C2,","place":["Canada"]}]},{"given":"S Cenk","family":"Sahinalp","sequence":"additional","affiliation":[{"name":"National Cancer Institute, NIH, 9000 Rockville Pike, Bethesda, MD, 20892,","place":["United States"]}]}],"member":"286","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"2024110904310845300_btae622-B1","doi-asserted-by":"crossref","first-page":"eaao2892","DOI":"10.1126\/sciimmunol.aao2892","article-title":"Inhibitory killer cell immunoglobulin-like receptors strengthen 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