{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T20:34:29Z","timestamp":1772138069857,"version":"3.50.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,11,4]],"date-time":"2024-11-04T00:00:00Z","timestamp":1730678400000},"content-version":"vor","delay-in-days":3,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Defective viral genomes (DVGs) are variants of the wild-type (wt) virus that lack the ability to complete autonomously an infectious cycle. However, in the presence of their parental (helper) wt virus, DVGs can interfere with the replication, encapsidation, and spread of functional genomes, acting as a significant selective force in viral evolution. DVGs also affect the host\u2019s immune responses and are linked to chronic infections and milder symptoms. Thus, identifying and characterizing DVGs is crucial for understanding infection prognosis. Quantifying DVGs is challenging due to their inability to sustain themselves, which makes it difficult to distinguish them from the helper virus, especially using high-throughput RNA sequencing. An accurate quantification is essential for understanding their very dynamical interactions with the helper virus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We present a method to simultaneously estimate the abundances of DVGs and wt genomes within a sample by identifying genomic regions with significant deviations from the expected sequencing depth. Our approach involves reconstructing the depth profile through a linear system of equations, which provides an estimate of the number of wt and DVG genomes of each type. Until now, in silico methods have only estimated the DVG-to-wt ratio for localized genomic regions. This is the first method that simultaneously estimates the proportions of wt and DVGs genome wide from short-reads RNA sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>The Matlab code and the synthetic datasets are freely available at https:\/\/github.com\/jmusan\/wtDVGquantific.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btae651","type":"journal-article","created":{"date-parts":[[2024,10,29]],"date-time":"2024-10-29T09:16:06Z","timestamp":1730193366000},"source":"Crossref","is-referenced-by-count":2,"title":["Quantifying defective and wild-type viruses from high-throughput RNA sequencing"],"prefix":"10.1093","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5570-4983","authenticated-orcid":false,"given":"Juan C","family":"Mu\u00f1oz-S\u00e1nchez","sequence":"first","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de Val\u00e8ncia , Paterna, Val\u00e8ncia 46980,","place":["Spain"]},{"name":"Departament de F\u00edsica Te\u00f2rica, Universitat de Val\u00e8ncia , Burjassot, Val\u00e8ncia 46100,","place":["Spain"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8570-424X","authenticated-orcid":false,"given":"Mar\u00eda J","family":"Olmo-Uceda","sequence":"additional","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de Val\u00e8ncia , Paterna, Val\u00e8ncia 46980,","place":["Spain"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1682-5798","authenticated-orcid":false,"given":"Jos\u00e9-\u00c1ngel","family":"Oteo","sequence":"additional","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de Val\u00e8ncia , Paterna, Val\u00e8ncia 46980,","place":["Spain"]},{"name":"Departament de F\u00edsica Te\u00f2rica, Universitat de Val\u00e8ncia , Burjassot, Val\u00e8ncia 46100,","place":["Spain"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8249-5593","authenticated-orcid":false,"given":"Santiago F","family":"Elena","sequence":"additional","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio), CSIC-Universitat de Val\u00e8ncia , Paterna, Val\u00e8ncia 46980,","place":["Spain"]},{"name":"Santa Fe Institute , Santa Fe, NM 87501,","place":["United States"]}]}],"member":"286","published-online":{"date-parts":[[2024,11,4]]},"reference":[{"key":"2024112216311226600_btae651-B1","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1261\/rna","article-title":"VODKA2: a fast and accurate method to detect non-standard viral genomes from large RNA-seq data sets","volume":"30","author":"Achouri","year":"2024","journal-title":"RNA"},{"key":"2024112216311226600_btae651-B2","volume-title":"Walsh Functions and their 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