{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T11:52:50Z","timestamp":1769601170868,"version":"3.49.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":4,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["874735"],"award-info":[{"award-number":["874735"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["101046203"],"award-info":[{"award-number":["101046203"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Accurate pathogen identification is crucial during outbreaks, especially with the emergence of new variants requiring frequent primer updates. However, resources for maintaining up-to-date verification of primer sequences are often limited, which poses challenges for reliable diagnosis and hinders potential monitoring efforts based on genome sequencing. To address this, we introduce ViralPrimer, a web server facilitating primer design, SARS-CoV-2 and Mpox variant monitoring, and adaptation to future threats. ViralPrimer aims to enhance diagnostic accuracy with its comprehensive primer database, mutation analysis assistance, and user primer upload feature. Its adaptable design allows monitoring of other rapidly mutating pathogens, contributing to broader public health protection efforts.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>ViralPrimer is freely accessible and open to all users with no login requirement at https:\/\/viralprimer.elte.hu\/. The application is hosted on a DJANGO v3.2.13 web server, with a PostgreSQL database, and the frontend was implemented using jQuery v3.6.0, vanilla JavaScript vES6, and Bootstrap v5.1.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btae657","type":"journal-article","created":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T14:41:20Z","timestamp":1730817680000},"source":"Crossref","is-referenced-by-count":1,"title":["ViralPrimer: a web server to monitor viral nucleic acid amplification tests\u2019 primer efficiency during pandemics, with emphasis on SARS-CoV-2 and Mpox"],"prefix":"10.1093","volume":"40","author":[{"given":"Norbert","family":"Deutsch","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, ELTE E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest 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H-1117,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orsolya Anna","family":"Pipek","sequence":"additional","affiliation":[{"name":"Department of Physics of Complex Systems, Institute of Physics and Astronomy, ELTE E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest H-1117,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f3zsef","family":"St\u00e9ger","sequence":"additional","affiliation":[{"name":"Department of Physics of Complex Systems, Institute of Physics and Astronomy, ELTE E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest H-1117,","place":["Hungary"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kriszti\u00e1n","family":"Papp","sequence":"additional","affiliation":[{"name":"Department of Physics of Complex Systems, Institute of Physics and Astronomy, ELTE E\u00f6tv\u00f6s Lor\u00e1nd University , Budapest 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