{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:14:27Z","timestamp":1787008467332,"version":"build-2736575974"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T00:00:00Z","timestamp":1737417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State Ministry of Science"},{"name":"Open Access Publication Fund of the University of Wuerzburg"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Summary<\/jats:title>\n                    <jats:p>We introduce software for reading, writing and processing fluorescence single-molecule and image spectroscopy data and developing analysis pipelines to unify various spectroscopic analysis tools. Our software can be used for processing multiple experiment types, e.g. for time-resolved single-molecule spectroscopy, laser scanning microscopy, fluorescence correlation spectroscopy and image correlation spectroscopy. The software is file format agnostic and processes multiple time-resolved data formats and outputs. Our software eliminates the need for data conversion and mitigates data archiving issues.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implementation<\/jats:title>\n                    <jats:p>tttrlib is available via pip (https:\/\/pypi.org\/project\/tttrlib\/) and bioconda while the open-source code is available via GitHub (https:\/\/github.com\/fluorescence-tools\/tttrlib). Presented examples and additional documentation demonstrating how to implement in vitro and live-cell image spectroscopy analysis are available at https:\/\/docs.peulen.xyz\/tttrlib and https:\/\/zenodo.org\/records\/14002224.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaf025","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T06:28:14Z","timestamp":1737095294000},"source":"Crossref","is-referenced-by-count":11,"title":["tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling"],"prefix":"10.1093","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-9755","authenticated-orcid":false,"given":"Thomas-Otavio","family":"Peulen","sequence":"first","affiliation":[{"name":"Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and Quantitative Biosciences Institute, University of California , San Francisco, CA, 94143,","place":["United States"]},{"name":"Rudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-University W\u00fcrzburg (JMU) , W\u00fcrzburg, 97080,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1852-6513","authenticated-orcid":false,"given":"Katherina","family":"Hemmen","sequence":"additional","affiliation":[{"name":"Rudolf Virchow Center for Integrative and Translational Bioimaging, Julius-Maximilians-University W\u00fcrzburg (JMU) , W\u00fcrzburg, 97080,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1350-9706","authenticated-orcid":false,"given":"Annemarie","family":"Greife","sequence":"additional","affiliation":[{"name":"Chair of Molecular Physical Chemistry, Heinrich-Heine University , D\u00fcsseldorf, 40225,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-4540","authenticated-orcid":false,"given":"Benjamin M","family":"Webb","sequence":"additional","affiliation":[{"name":"Department of Bioengineering and Therapeutic Sciences, Department of Pharmaceutical Chemistry, and Quantitative Biosciences Institute, University of California , San Francisco, CA, 94143,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0554-4520","authenticated-orcid":false,"given":"Suren","family":"Felekyan","sequence":"additional","affiliation":[{"name":"Chair of Molecular Physical Chemistry, Heinrich-Heine University , D\u00fcsseldorf, 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