{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:50:51Z","timestamp":1759333851576,"version":"build-2065373602"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":17,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Federation Hospitalo-Universitaire FHU G4 Genomics Caen-Rouen-Lille-Amiens"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Summary<\/jats:title>\n                  <jats:p>Functional testing of RNA using minigene splicing assays is increasingly being realized to demonstrate the effects of variants on splicing. In complex cases, variant pathogenicity is assessed by Sanger sequencing, which can be time consuming and may be replaced by short read sequencing. Moreover, strategies based on long read sequencing of the amplified minigene construct are promising and allow the isoforms to be fully characterized. We introduce MAGIC, a user-friendly tool that first generates the artificial construction genome files required to then perform alignment, assembly and annotation of the isoforms obtained by either short or long read minigene splicing assay sequencing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Availability and implementation<\/jats:title>\n                  <jats:p>MAGIC is available at https:\/\/github.com\/LBGC-CFB\/MAGIC. Zenodo DOI: 10.5281\/zenodo.17052752.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaf525","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T00:28:22Z","timestamp":1758328102000},"source":"Crossref","is-referenced-by-count":0,"title":["Decipher RNA isoform combinations from minigene splicing assays and massive parallel sequencing with MAGIC"],"prefix":"10.1093","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3929-1513","authenticated-orcid":false,"given":"Camille","family":"Aucouturier","sequence":"first","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]},{"name":"Inserm U1245, Cancer and Brain Genomics, FHU G4 Genomics, Normandie University , Rouen 76183,","place":["France"]},{"name":"Normandie Univ, UNICAEN , Caen 14000,","place":["France"]}]},{"given":"Nicolas","family":"Goardon","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]},{"name":"Inserm U1245, Cancer and Brain Genomics, FHU G4 Genomics, Normandie University , Rouen 76183,","place":["France"]}]},{"given":"Laurent","family":"Cast\u00e9ra","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]},{"name":"Inserm U1245, Cancer and Brain Genomics, FHU G4 Genomics, Normandie University , Rouen 76183,","place":["France"]}]},{"given":"Alexandre","family":"Atkinson","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]}]},{"given":"Thibaut","family":"Lavol\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]}]},{"given":"Ang\u00e9lina","family":"Legros","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]}]},{"given":"Agathe","family":"Ricou","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie et de g\u00e9n\u00e9tique du cancer, D\u00e9partement de Biopathologie, Centre Fran\u00e7ois Baclesse , Caen 14000,","place":["France"]},{"name":"Inserm U1245, Cancer and Brain Genomics, FHU G4 Genomics, Normandie University , Rouen 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