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Integrating multiple mIF panels through image alignment of serial slide significantly expands the number of cell populations analysed in a single space. IntegrAlign was developed to optimize this integration on serial whole slides, enhancing the value and applicability of -mIF for comprehensive spatial analyses and enabling biomarker discovery at scale.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>IntegrAlign, leveraging the SimpleITK toolkit, applies a two-step alignment using rigid and B-spline transformations to integrate serial mIF whole slides. Validation on simulated and real datasets demonstrated alignment accuracy below the diameter of a cell nucleus (\u223c6\u2009\u00b5m), outperforming existing methods. This precision enhances spatial analyses by combining extended phenotypic data, supporting novel insights into tissue architecture and cellular interactions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability and implemention<\/jats:title>\n                    <jats:p>IntegrAlign is open-source, implemented in Python, and freely available under the MIT license at https:\/\/github.com\/CAUXlab\/IntegrAlign.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btaf567","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:27:32Z","timestamp":1760531252000},"source":"Crossref","is-referenced-by-count":0,"title":["IntegrAlign: a comprehensive tool for multi-immunofluorescence panel integration through image alignment"],"prefix":"10.1093","volume":"41","author":[{"given":"Leo","family":"Hermet","sequence":"first","affiliation":[{"name":"Centre L\u00e9on B\u00e9rard, 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