{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T17:46:39Z","timestamp":1782409599629,"version":"3.54.5"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":8,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Valinet","award":["PID2021-128865NB-I00"],"award-info":[{"award-number":["PID2021-128865NB-I00"]}]},{"name":"Metacell","award":["PID2024-163039NB-I00"],"award-info":[{"award-number":["PID2024-163039NB-I00"]}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011033","name":"Agencia Estatal de Investigaci\u00f3n","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"Fondo Europeo de Desarrollo Regional","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"name":"PROMETEO","award":["PROMETEO\/2021\/056\u201301"],"award-info":[{"award-number":["PROMETEO\/2021\/056\u201301"]}]},{"DOI":"10.13039\/501100003359","name":"Generalitat Valenciana","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Grapedia COST innovators","award":["IG17111"],"award-info":[{"award-number":["IG17111"]}]},{"name":"Grapedia COST innovators","award":["IEC-BG1-2023\u20134"],"award-info":[{"award-number":["IEC-BG1-2023\u20134"]}]},{"DOI":"10.13039\/501100010770","name":"Institute for Catalan Studies","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100010770","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Motivation<\/jats:title>\n                    <jats:p>Genome annotation files (GFF3\/GTF) are the standard for storing genomic feature data, yet their flexibility often results in formatting inconsistencies that create bottlenecks for downstream bioinformatics analyses. A robust, unified framework is required to parse, standardise, and validate these files to ensure interoperability and facilitate complex comparative genomic tasks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We present AEGIS (Annotation Extraction and Genomic Integration Suite), a comprehensive toolkit designed to parse, correct, and standardise genome annotations. Beyond quality control, AEGIS provides advanced modules for flexible feature extraction (e.g., coding sequences, promoters) and comparative genomic analysis. Uniquely, it integrates multiple lines of evidence, including sequence homology, synteny, and coordinate-based lift-overs, to assess gene model correspondence and infer orthology. We demonstrate the utility of AEGIS by quantifying complex structural changes between Arabidopsis annotation versions and identifying high-confidence orthologues across diverse plant genomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Availability<\/jats:title>\n                    <jats:p>AEGIS is implemented in Python. Source code and documentation are freely available under the GPL-3 license at https:\/\/github.com\/Tomsbiolab\/aegis and as a Docker container at https:\/\/hub.docker.com\/r\/tomsbiolab\/aegis. The package is also available on PyPI (pip install aegis-bio).<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/bioinformatics\/btag363","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T11:32:58Z","timestamp":1781004778000},"source":"Crossref","is-referenced-by-count":0,"title":["AEGIS: an annotation extraction and genomic integration resource"],"prefix":"10.1093","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1623-3039","authenticated-orcid":false,"given":"David","family":"Navarro-Pay\u00e1","sequence":"first","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio, CSIC-UV) , Valencia, 46980,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9180-3241","authenticated-orcid":false,"given":"Antonio","family":"Santiago","sequence":"additional","affiliation":[{"name":"Institute for Integrative Systems Biology (I2SysBio, CSIC-UV) , Valencia, 46980,","place":["Spain"]},{"name":"Programa de Doctorado en Biotecnolog\u00eda, Universitat Polit\u00e8cnica de Val\u00e8ncia , Valencia,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Velt","sequence":"additional","affiliation":[{"name":"INRAE-Universit\u00e9 de Strasbourg, SVQV , Colmar, 68000,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4555-7243","authenticated-orcid":false,"given":"Marco","family":"Moretto","sequence":"additional","affiliation":[{"name":"Unit of Digital Agriculture, Research and Innovation Centre, Fondazione Edmund Mach , San Michele all\u2019Adige, 38098,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5355-3408","authenticated-orcid":false,"given":"Camille","family":"Rustenholz","sequence":"additional","affiliation":[{"name":"INRAE-Universit\u00e9 de Strasbourg, SVQV , 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