{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:20:33Z","timestamp":1773289233414,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Data from one-channel cDNA microarray studies may exhibit poor reproducibility due to spatial heterogeneity, non-linear array-to-array variation and problems in correcting for background. Uncorrected, these phenomena can give rise to misleading conclusions.<\/jats:p>\n               <jats:p>Results: Spatial heterogeneity may be corrected using two-dimensional loess smoothing (Colantuoni et al., 2002). Non-linear between-array variation may be corrected using an iterative application of one-dimensional loess smoothing. A method for background correction using a smoothing function rather than simple subtraction is described. These techniques promote within-array spatial uniformity and between-array reproducibility. Their application is illustrated using data from a study of the effects of an insulin sensitizer, rosiglitazone, on gene expression in white adipose tissue in diabetic db\/db mice. They may also be useful with data from two-channel cDNA microarrays and from oligonucleotide arrays.<\/jats:p>\n               <jats:p>Availability: R functions for the methods described are available on request from the author.<\/jats:p>\n               <jats:p>Contact: DEd@novonordisk.com<\/jats:p>","DOI":"10.1093\/bioinformatics\/btg083","type":"journal-article","created":{"date-parts":[[2003,4,30]],"date-time":"2003-04-30T19:43:50Z","timestamp":1051731830000},"page":"825-833","source":"Crossref","is-referenced-by-count":106,"title":["Non-linear normalization and background correction in\none-channel cDNA microarray studies"],"prefix":"10.1093","volume":"19","author":[{"given":"David","family":"Edwards","sequence":"first","affiliation":[{"name":"Department of Biostatistics, Novo Nordisk, Bagsvaerd Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2003,5,1]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/19\/7\/825\/48904323\/bioinformatics_19_7_825.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/19\/7\/825\/48904323\/bioinformatics_19_7_825.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T17:23:10Z","timestamp":1674667390000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/19\/7\/825\/197375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,1]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,5,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btg083","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2003,5,1]]},"published":{"date-parts":[[2003,5,1]]}}}