{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,6]],"date-time":"2024-06-06T21:14:01Z","timestamp":1717708441489},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,2,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: SEPON, Selection and Evaluation Pipeline for OligoNucleotide generates n-mer oligonucleotide sequences from expressed sequence tags of non-annotated genomes for microarray gene-expression profiling. A non-target melting temperature (Tm) algorithm will reduce cross-hybridization by estimating Tm of oligonucleotide hybridization to non-specific targets (non-target Tm) and discard oligonucleotides with non-target Tm estimate above user-defined threshold. SEPON allows user-defined filtering, predicts exon location, assigns penalty based on 3\u2032 distance, GC content, secondary structure Tm and non-target Tm and ranks oligonucleotides for optimal selection.<\/jats:p>\n               <jats:p>Availability: The program is freely available for non-commercial and academic use under the GNU General Public License and is obtained upon request to authors.<\/jats:p>\n               <jats:p>Supplementary information: \u00a0http:\/\/www.agrsci.dk\/hag\/sepon\/<\/jats:p>","DOI":"10.1093\/bioinformatics\/btg434","type":"journal-article","created":{"date-parts":[[2004,2,11]],"date-time":"2004-02-11T16:52:44Z","timestamp":1076518364000},"page":"428-429","source":"Crossref","is-referenced-by-count":9,"title":["SEPON, a Selection and Evaluation Pipeline for OligoNucleotides based on ESTs with a non-target <i>T<\/i>m algorithm for reducing cross-hybridization in microarray gene expression experiments"],"prefix":"10.1093","volume":"20","author":[{"given":"Henrik","family":"Hornsh\u00f8j","sequence":"first","affiliation":[]},{"given":"Henrik","family":"Stengaard","sequence":"additional","affiliation":[]},{"given":"Frank","family":"Panitz","sequence":"additional","affiliation":[]},{"given":"Christian","family":"Bendixen","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2004,1,22]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/3\/428\/48905089\/bioinformatics_20_3_428.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/3\/428\/48905089\/bioinformatics_20_3_428.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T18:19:35Z","timestamp":1674670775000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/20\/3\/428\/186380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1,22]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,2,12]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btg434","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2004,2,12]]},"published":{"date-parts":[[2004,1,22]]}}}