{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T03:26:29Z","timestamp":1764905189830},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,6,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Multiple alignment of highly divergent sequences is a challenging problem for which available programs tend to show poor performance. Generally, this is due to a scoring function that does not describe biological reality accurately enough or a heuristic that cannot explore solution space efficiently enough. In this respect, we present a new program, Align-m, that uses a non-progressive local approach to guide a global alignment.<\/jats:p>\n               <jats:p>Results: Two large test sets were used that represent the entire SCOP classification and cover sequence similarities between 0 and 50% identity. Performance was compared with the publicly available algorithms ClustalW, T-Coffee and DiAlign. In general, Align-m has comparable or slightly higher accuracy in terms of correctly aligned residues, especially for distantly related sequences. Importantly, it aligns much fewer residues incorrectly, with average differences of over 15% compared with some of the other algorithms.<\/jats:p>\n               <jats:p>Availability: Align-m and the test sets are available at http:\/\/bioinformatics.vub.ac.be<\/jats:p>","DOI":"10.1093\/bioinformatics\/bth116","type":"journal-article","created":{"date-parts":[[2004,3,2]],"date-time":"2004-03-02T21:41:06Z","timestamp":1078263666000},"page":"1428-1435","source":"Crossref","is-referenced-by-count":63,"title":["Align-m\u2014a new algorithm for multiple alignment of highly divergent sequences"],"prefix":"10.1093","volume":"20","author":[{"given":"Ivo","family":"Van Walle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ignace","family":"Lasters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lode","family":"Wyns","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,2,12]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/9\/1428\/48905457\/bioinformatics_20_9_1428.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/9\/1428\/48905457\/bioinformatics_20_9_1428.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T16:54:59Z","timestamp":1674665699000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/20\/9\/1428\/195628"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,12]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2004,6,12]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bth116","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2004,6,12]]},"published":{"date-parts":[[2004,2,12]]}}}