{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T13:29:13Z","timestamp":1773754153773,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Before performing a polymerase chain reaction experiment, a pair of primers to clip the target DNA subsequence is required. However, this is a tedious task as too many constraints need to be satisfied. Various kinds of approaches for designing a primer have been proposed in the last few decades, but most of them do not have restriction sites on the designed primers and do not satisfy the specificity constraint.<\/jats:p>\n               <jats:p>Results: The proposed algorithm imitates nature's process of evolution and genetic operations on chromosomes in order to achieve optimal solutions, and is a best fit for DNA behavior. Experimental results indicate that the proposed algorithm can find a pair of primers that not only obeys the design properties but also has a specific restriction site and specificity. Gel electrophoresis verifies that the proposed method really can clip out the target sequence.<\/jats:p>\n               <jats:p>Availability: A public version of the software is available on request from the authors.<\/jats:p>","DOI":"10.1093\/bioinformatics\/bth147","type":"journal-article","created":{"date-parts":[[2004,3,2]],"date-time":"2004-03-02T21:41:06Z","timestamp":1078263666000},"page":"1710-1717","source":"Crossref","is-referenced-by-count":48,"title":["Primer design using genetic algorithm"],"prefix":"10.1093","volume":"20","author":[{"given":"Jain-Shing","family":"Wu","sequence":"first","affiliation":[]},{"given":"Chungnan","family":"Lee","sequence":"additional","affiliation":[]},{"given":"Chien-Chang","family":"Wu","sequence":"additional","affiliation":[]},{"given":"Yow-Ling","family":"Shiue","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2004,2,26]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/11\/1710\/48905910\/bioinformatics_20_11_1710.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/11\/1710\/48905910\/bioinformatics_20_11_1710.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T16:47:21Z","timestamp":1674665241000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/20\/11\/1710\/300143"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,2,26]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,7,22]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bth147","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2004,7,22]]},"published":{"date-parts":[[2004,2,26]]}}}