{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T07:31:02Z","timestamp":1771054262577,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: To facilitate the designing process for vector-based siRNA and siRNA cassette, a tool set has been developed consisting of a siRNA target finder, a siRNA construct builder and a siRNA sequence scrambler. The siRNA target finder is used to identify candidate siRNA target sites. The program automates homology filtering, minimizes non-specific cross-reaction, filters target sites based on RNA duplex internal stability and siRNA sense\/anti-sense strand secondary structure. The siRNA construct builder is used to create a siRNA hairpin construct as a vector insert or a cassette insert. The siRNA sequence scrambler is used to generate a negative control sequence for siRNA experiments. Together, these programs provide a comprehensive utility set to address the specific bioinformatics need of DNA-based siRNA design.<\/jats:p>\n               <jats:p>Availability: The Web-based design tools are available at GenScript Website (siRNA design center). http:\/\/www.genscript.com or http:\/\/www.genscript.com\/rnai.html#design or http:\/\/www.genscript.com\/sirna_ca.html#design<\/jats:p>","DOI":"10.1093\/bioinformatics\/bth164","type":"journal-article","created":{"date-parts":[[2004,3,9]],"date-time":"2004-03-09T01:24:47Z","timestamp":1078795487000},"page":"1818-1820","source":"Crossref","is-referenced-by-count":44,"title":["A Web-based design center for vector-based siRNA and siRNA cassette"],"prefix":"10.1093","volume":"20","author":[{"given":"Luquan","family":"Wang","sequence":"first","affiliation":[{"name":"GenScript Corporation, 120 Centennial Avenue, Piscataway, NJ 08854, USA"}]},{"given":"Forest Y.","family":"Mu","sequence":"additional","affiliation":[{"name":"GenScript Corporation, 120 Centennial Avenue, Piscataway, NJ 08854, USA"}]}],"member":"286","published-online":{"date-parts":[[2004,7,22]]},"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/11\/1818\/48905860\/bioinformatics_20_11_1818.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/20\/11\/1818\/48905860\/bioinformatics_20_11_1818.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T16:46:05Z","timestamp":1674665165000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/20\/11\/1818\/300221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,22]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,7,22]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bth164","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2004,7,22]]},"published":{"date-parts":[[2004,7,22]]}}}