{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T09:20:08Z","timestamp":1786526408617,"version":"build-2736575974"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: We present here a neural network based method for prediction of N-terminal acetylation\u2014by far the most abundant post-translational modification in eukaryotes. The method was developed on a yeast dataset for N-acetyltransferase A (NatA) acetylation, which is the type of N-acetylation for which most examples are known and for which orthologs have been found in several eukaryotes. We obtain correlation coefficients close to 0.7 on yeast data and a sensitivity up to 74% on mammalian data, suggesting that the method is valid for eukaryotic NatA orthologs.<\/jats:p>\n               <jats:p>Availability: The NetAcet prediction method is available as a public web server at http:\/\/www.cbs.dtu.dk\/services\/NetAcet\/<\/jats:p>\n               <jats:p>Contact: \u00a0nikob@cbs.dtu.dk<\/jats:p>\n               <jats:p>Supplementary information: \u00a0http:\/\/www.cbs.dtu.dk\/services\/NetAcet\/<\/jats:p>","DOI":"10.1093\/bioinformatics\/bti130","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T01:14:59Z","timestamp":1100222099000},"page":"1269-1270","source":"Crossref","is-referenced-by-count":120,"title":["NetAcet: prediction of N-terminal acetylation sites"],"prefix":"10.1093","volume":"21","author":[{"given":"Lars","family":"Kiemer","sequence":"first","affiliation":[{"name":"Center for Biological Sequence Analysis, BioCentrum-DTU Building 208 Technical University of Denmark DK-2800 Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jannick Dyrl\u00f8v","family":"Bendtsen","sequence":"additional","affiliation":[{"name":"Center for Biological Sequence Analysis, BioCentrum-DTU Building 208 Technical University of Denmark DK-2800 Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaj","family":"Blom","sequence":"additional","affiliation":[{"name":"Center for Biological Sequence Analysis, BioCentrum-DTU Building 208 Technical University of Denmark DK-2800 Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2004,11,11]]},"reference":[{"key":"2023013107282875400_B1","doi-asserted-by":"crossref","unstructured":"Apweiler, R., Bairoch, A., Wu, C.H., Barker, W.C., Boeckmann, B., Ferro, S., Gasteiger, E., Huang, H., Lopez, R., Magrane, M., et al. 2004Uniprot: the universal protein knowledgebase. Nucleic Acids Res.32D115\u2013D119","DOI":"10.1093\/nar\/gkh131"},{"key":"2023013107282875400_B2","doi-asserted-by":"crossref","unstructured":"Augen, J. and Wold, F. 1986How much sequence information is needed for the regulation of amino-terminal acetylation of eukaryotic proteins?. Trends Biochem. Sci.11494\u2013497","DOI":"10.1016\/0968-0004(86)90075-7"},{"key":"2023013107282875400_B3","doi-asserted-by":"crossref","unstructured":"Blom, N., Hansen, J., Blaas, D., Brunak, S. 1996Cleavage site analysis in picornaviral polyproteins: discovering cellular targets by neural networks. Protein Sci.52203\u20132216","DOI":"10.1002\/pro.5560051107"},{"key":"2023013107282875400_B4","doi-asserted-by":"crossref","unstructured":"Cover, T.M. and Thomas, J.A. Elements of Information Theory.1991, New York  John Wiley and Sons, Inc","DOI":"10.1002\/0471200611"},{"key":"2023013107282875400_B5","doi-asserted-by":"crossref","unstructured":"Matthews, B.W. 1975Comparison of the predicted and observed secondary structure of T4 phage lysozyme. Biochim. Biophys. Acta.405,  pp. 442\u2013451","DOI":"10.1016\/0005-2795(75)90109-9"},{"key":"2023013107282875400_B6","doi-asserted-by":"crossref","unstructured":"Nielsen, H., Engelbrecht, J., Brunak, S., von Heijne, G. 1997Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng.101\u20136","DOI":"10.1093\/protein\/10.1.1"},{"key":"2023013107282875400_B7","doi-asserted-by":"crossref","unstructured":"Perrot, M., Sagliocco, F., Mini, T., Monribot, C., Schneider, U., Shevchenko, A., Mann, M., Jeno, P., Boucherie, H. 1999Two-dimensional gel protein database of saccharomyces cerevisiae (update 1999). Electrophoresis202280\u20132298","DOI":"10.1002\/(SICI)1522-2683(19990801)20:11<2280::AID-ELPS2280>3.0.CO;2-Q"},{"key":"2023013107282875400_B8","doi-asserted-by":"crossref","unstructured":"Polevoda, B. and Sherman, F. 2000N\u03b1-terminal acetylation of eukaryotic proteins. J. Biol. Chem.27536479\u201336482","DOI":"10.1074\/jbc.R000023200"},{"key":"2023013107282875400_B9","doi-asserted-by":"crossref","unstructured":"Polevoda, B. and Sherman, F. 2003N-terminal acetyltransferases and sequence requirements for N-terminal acetylation of eukaryotic proteins. J. Mol. Biol.325595\u2013622","DOI":"10.1016\/S0022-2836(02)01269-X"},{"key":"2023013107282875400_B10","doi-asserted-by":"crossref","unstructured":"Schneider, T.D. and Stephens, R.M. 1990Sequence logos: a new way to display consensus sequences. Nucleic Acids Res.186097\u20136100","DOI":"10.1093\/nar\/18.20.6097"},{"key":"2023013107282875400_B11","doi-asserted-by":"crossref","unstructured":"Shannon, C.E. 1948A mathematical theory of communication. Bell System Tech. J.27379\u2013423 623\u2013656","DOI":"10.1002\/j.1538-7305.1948.tb00917.x"},{"key":"2023013107282875400_B12","doi-asserted-by":"crossref","unstructured":"Thompson, J.D., Higgins, D.G., Gibson, T.J. 1994CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res.224673\u20134680","DOI":"10.1093\/nar\/22.22.4673"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/21\/7\/1269\/48966924\/bioinformatics_21_7_1269.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/21\/7\/1269\/48966924\/bioinformatics_21_7_1269.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T10:05:50Z","timestamp":1675159550000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/21\/7\/1269\/269004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,11]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bti130","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2005,4,1]]},"published":{"date-parts":[[2004,11,11]]}}}