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Primaclade creates an HTML results page that recaps the original alignment, provides a consensus sequence and lists primers for each alignment area, with primers color-coded to reflect the level of degeneracy in the primer.<\/jats:p>\n               <jats:p>Availability: Primaclade can be accessed freely at http:\/\/www.umsl.edu\/~biology\/Kellogg\/primaclade.html<\/jats:p>\n               <jats:p>Contact: \u00a0tkellogg@umsl.edu<\/jats:p>","DOI":"10.1093\/bioinformatics\/bti134","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T01:14:59Z","timestamp":1100222099000},"page":"1263-1264","source":"Crossref","is-referenced-by-count":109,"title":["Primaclade\u2014a flexible tool to find conserved PCR primers across multiple species"],"prefix":"10.1093","volume":"21","author":[{"given":"Michael D.","family":"Gadberry","sequence":"first","affiliation":[{"name":"Department of Biology, University of Missouri-St. Louis One University Boulevard, St. Louis, MO 63121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon T.","family":"Malcomber","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Missouri-St. Louis One University Boulevard, St. Louis, MO 63121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew N.","family":"Doust","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Missouri-St. Louis One University Boulevard, St. Louis, MO 63121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Kellogg","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Missouri-St. Louis One University Boulevard, St. Louis, MO 63121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,11,11]]},"reference":[{"key":"2023013107282221500_B1","doi-asserted-by":"crossref","unstructured":"Fielding, R.T. and Kaiser, G. 1997The Apache HTTP Server Project. 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