{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T07:24:43Z","timestamp":1773473083938,"version":"3.50.1"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: The translation start site plays an important role in the control of translation efficiency of eukaryotic mRNAs. However, mRNAs with a suboptimal context of start AUG codon are relatively abundant. It is likely that at least some mRNAs with suboptimal start codon context contain the other signals providing additional information for efficient AUG recognition.<\/jats:p>\n               <jats:p>Results: Frequency of AUG codons at the beginning of the coding part of eukaryotic mRNAs was analyzed in relation to the context of translation start codon. It was found that the observed downstream AUG content in the mRNAs with optimal start codon context was close to the expected value, whereas it was significantly higher in the mRNAs with a suboptimal context. It is likely that downstream AUG codons can often be utilized as additional start sites to increase translation rate of mRNAs with a suboptimal context of the annotated start codon and many eukaryotic proteins can be characterized by some N-end heterogeneity.<\/jats:p>\n               <jats:p>Contact: \u00a0ak@bionet.nsc.ru<\/jats:p>","DOI":"10.1093\/bioinformatics\/bti136","type":"journal-article","created":{"date-parts":[[2004,11,6]],"date-time":"2004-11-06T01:14:14Z","timestamp":1099703654000},"page":"837-840","source":"Crossref","is-referenced-by-count":37,"title":["AUG codons at the beginning of protein coding sequences are frequent in eukaryotic mRNAs with a suboptimal start codon context"],"prefix":"10.1093","volume":"21","author":[{"given":"Alex V.","family":"Kochetov","sequence":"first","affiliation":[{"name":"Institute of Cytology and Genetics Lavrentieva 10, Novosibirsk 630090 Russia and Novosibirsk State University Novosibirsk 630090, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2004,11,5]]},"reference":[{"key":"2023013107420517300_B1","doi-asserted-by":"crossref","unstructured":"Casadei, R., Strippoli, P., D'Addabbo, P., Canaider, S., Lenzi, L., Vitale, L., Giannone, S., Frabetti, F., Facchin, F., Carinci, P., Zannotti, M. 2003mRNA 5\u2032 region sequence incompleteness: a potential source of systematic errors in translation initiation codon assignment in human mRNAs. 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Bioinformatics17890\u2013900","DOI":"10.1093\/bioinformatics\/17.10.890"},{"key":"2023013107420517300_B10","doi-asserted-by":"crossref","unstructured":"Sawant, S.V., Kiran, K., Singh, P.K., Tuli, R. 2001Sequence architecture downstream of the initiator codon enhances gene expression and protein stability in plants. Plant Physiol.1261630\u20131636","DOI":"10.1104\/pp.126.4.1630"},{"key":"2023013107420517300_B11","doi-asserted-by":"crossref","unstructured":"Shabalina, S.A., Ogurtsov, A.Y., Rogozin, I.B., Koonin, E.V., Lipman, D.J. 2004Comparative analysis of orthologous eukaryotic mRNAs: potential hidden functional signals. Nucleic Acids Res.321774\u20131782","DOI":"10.1093\/nar\/gkh313"},{"key":"2023013107420517300_B12","unstructured":"Wang, X. and -Q. and Rothnagel, J.A. 20045\u2032-untranslated regions with multiple upstream AUG codons can support low-level translation via leaky scanning and reinitiation. 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