{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T20:35:53Z","timestamp":1762374953596},"reference-count":9,"publisher":"Oxford University Press (OUP)","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: WebACT is an online resource which enables the rapid provision of simultaneous BLAST comparisons between up to five genomic sequences in a format amenable for visualization with the well-known Artemis Comparison Tool (ACT). Comparisons can be generated on-the-fly using sequences directly retrieved via EMBL database queries, or by entering or uploading user sequences. Furthermore, pre-computed comparisons are available between all publicly available, completed prokaryotic genomes and plasmids currently contained within the Genome Reviews database (372 sequences, representing 175 different species). The system is designed to minimize the volume of downloaded data and maximize performance. Genome sequences, annotation and pre-computed comparisons are stored in a relational database allowing flexible querying based on user-defined sequence regions, from whole genome to a defined region flanking a specified gene. Comparison and sequence files, whether computed online or retrieved from the database of pre-computed genome comparisons, can be viewed online using ACT and are available for download.<\/jats:p>\n               <jats:p>Availability: Freely accessible at http:\/\/www.webact.org<\/jats:p>\n               <jats:p>Contact: \u00a0admin@webact.org<\/jats:p>\n               <jats:p>Supplementary information: User guide and worked examples are available at http:\/\/www.webact.org\/WebACT\/docs<\/jats:p>","DOI":"10.1093\/bioinformatics\/bti601","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T02:43:46Z","timestamp":1123037026000},"page":"3665-3666","source":"Crossref","is-referenced-by-count":80,"title":["WebACT\u2014an online companion for the Artemis Comparison Tool"],"prefix":"10.1093","volume":"21","author":[{"given":"J. C.","family":"Abbott","sequence":"first","affiliation":[]},{"given":"D. M.","family":"Aanensen","sequence":"additional","affiliation":[]},{"given":"K.","family":"Rutherford","sequence":"additional","affiliation":[]},{"given":"S.","family":"Butcher","sequence":"additional","affiliation":[]},{"given":"B. G.","family":"Spratt","sequence":"additional","affiliation":[]}],"member":"286","published-online":{"date-parts":[[2005,8,2]]},"reference":[{"key":"2023060912063187100_B1","doi-asserted-by":"crossref","unstructured":"Altschul, S.F., et al. 1997Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res.253389\u20133402","DOI":"10.1093\/nar\/25.17.3389"},{"key":"2023060912063187100_B2","unstructured":"Bioinformatics Carver, T.J., et al. 2005ACT: The Artemis Comparison Tool.   in press"},{"key":"2023060912063187100_B3","doi-asserted-by":"crossref","unstructured":"Kanz, C., et al. 2005The EMBL Nucleotide Sequence Database. Nucleic Acids Res.33D29\u2013D33","DOI":"10.1093\/nar\/gki098"},{"key":"2023060912063187100_B4","doi-asserted-by":"crossref","unstructured":"Kersey, P., et al. 2005Integr8 and Genome Reviews: integrated views of complete genomes and proteomes. Nucleic Acids Res.33297\u2013302","DOI":"10.1093\/nar\/gki039"},{"key":"2023060912063187100_B5","unstructured":"Korf, I., et al. 2003BLAST.  , Sebastapol  O'Reilly and Associates"},{"key":"2023060912063187100_B6","doi-asserted-by":"crossref","unstructured":"Sonnhammer, E.L.L. and Durbin, R. 1994A workbench for large scale sequence homology analysis. Comput. Appl. Biosci.10301\u2013307","DOI":"10.1093\/bioinformatics\/10.3.301"},{"key":"2023060912063187100_B7","unstructured":"Stajich, J.E., et al. 2002The Bioperl Toolkit: Perl modules for the life sciences. Genome Res.101611\u20131618"},{"key":"2023060912063187100_B8","unstructured":"Schwartz, S., et al. 2000PipMaker\u2014a web server for aligning two genomic DNA sequences. Genome Res.10577\u2013586"},{"key":"2023060912063187100_B9","unstructured":"Schwartz, S., et al. 2003Human\u2013mouse alignments with BLASTZ. Genome Res13103\u2013107"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/21\/18\/3665\/50554883\/bioinformatics_21_18_3665.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/21\/18\/3665\/50554883\/bioinformatics_21_18_3665.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T12:07:09Z","timestamp":1686312429000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/21\/18\/3665\/202415"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8,2]]},"references-count":9,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2005,9,15]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/bti601","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2005,9]]},"published":{"date-parts":[[2005,8,2]]}}}