{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:57:20Z","timestamp":1792173440445,"version":"4.4.0"},"reference-count":15,"publisher":"Oxford University Press (OUP)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: We have developed a web server, iPTREE-STAB for discriminating the stability of proteins (stabilizing or destabilizing) and predicting their stability changes (\u0394\u0394G) upon single amino acid substitutions from amino acid sequence. The discrimination and prediction are mainly based on decision tree coupled with adaptive boosting algorithm, and classification and regression tree, respectively, using three neighboring residues of the mutant site along N- and C-terminals. Our method showed an accuracy of 82% for discriminating the stabilizing and destabilizing mutants, and a correlation of 0.70 for predicting protein stability changes upon mutations.<\/jats:p><jats:p>Availability: \u00a0http:\/\/bioinformatics.myweb.hinet.net\/iptree.htm<\/jats:p><jats:p>Contact: \u00a0michael-gromiha@aist.go.jp<\/jats:p><jats:p>Supplementary information: Dataset and other details are given.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btm100","type":"journal-article","created":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T00:18:49Z","timestamp":1174609129000},"page":"1292-1293","source":"Crossref","is-referenced-by-count":136,"title":["iPTREE-STAB: interpretable decision tree based method for predicting protein stability changes upon mutations"],"prefix":"10.1093","volume":"23","author":[{"given":"Liang-Tsung","family":"Huang","sequence":"first","affiliation":[{"name":"1 Department of Computer Science and Information Engineering, Ming-Dao University, Changhua 523, 2Institute of Information Engineering and Computer Science, Feng-Chia University, Taichung 407, Taiwan, 3Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tokyo Waterfront Bio-IT Research Building, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan and 4Department of Biological Science and Technology, and Institute of Bioinformatics, National Chiao-Tung University, Hsinchu 300, Taiwan"},{"name":"1 Department of Computer Science and Information Engineering, Ming-Dao University, Changhua 523, 2Institute of Information Engineering and Computer Science, Feng-Chia University, Taichung 407, Taiwan, 3Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tokyo Waterfront Bio-IT Research Building, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan and 4Department of Biological Science and Technology, and Institute of Bioinformatics, National Chiao-Tung University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Michael","family":"Gromiha","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science and Information Engineering, Ming-Dao University, Changhua 523, 2Institute of Information Engineering and Computer Science, Feng-Chia University, Taichung 407, Taiwan, 3Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tokyo Waterfront Bio-IT Research Building, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan and 4Department of Biological Science and Technology, and Institute of Bioinformatics, National Chiao-Tung University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinn-Ying","family":"Ho","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science and Information Engineering, Ming-Dao University, Changhua 523, 2Institute of Information Engineering and Computer Science, Feng-Chia University, Taichung 407, Taiwan, 3Computational Biology Research Center (CBRC), National Institute of Advanced Industrial Science and Technology (AIST), AIST Tokyo Waterfront Bio-IT Research Building, 2-42 Aomi, Koto-ku, Tokyo 135-0064, Japan and 4Department of Biological Science and Technology, and Institute of Bioinformatics, National Chiao-Tung University, Hsinchu 300, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,3,22]]},"reference":[{"key":"2023041104482742800_","doi-asserted-by":"crossref","first-page":"D120","DOI":"10.1093\/nar\/gkh082","article-title":"ProTherm, version 4.0: thermodynamic database for proteins and mutants","volume":"32","author":"Bava","year":"2004","journal-title":"Nucleic Acids Res."},{"key":"2023041104482742800_","volume-title":"Classification and regression trees","author":"Breiman","year":"1984"},{"key":"2023041104482742800_","doi-asserted-by":"crossref","first-page":"I63","DOI":"10.1093\/bioinformatics\/bth928","article-title":"A neural-network-based method for predicting protein stability changes upon single point mutations","volume":"20","author":"Capriotti","year":"2004","journal-title":"Bioinformatics"},{"key":"2023041104482742800_","doi-asserted-by":"crossref","first-page":"W306","DOI":"10.1093\/nar\/gki375","article-title":"I-Mutant2.0: predicting stability changes upon mutation from the protein sequence or structure","volume":"33","author":"Capriotti","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"2023041104482742800_","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1002\/prot.20810","article-title":"Prediction of protein stability changes for single-site mutations using support vector machines","volume":"62","author":"Cheng","year":"2006","journal-title":"Proteins"},{"key":"2023041104482742800_","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1006\/jcss.1997.1504","article-title":"A decision-theoretic generalization of on-line learning and an application to boosting","volume":"55","author":"Freund","year":"1997","journal-title":"J. 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