{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:27:19Z","timestamp":1761611239101,"version":"3.37.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":2804,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.0\/uk\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reverse phase protein arrays (RPPAs) are a powerful high-throughput tool for measuring protein concentrations in a large number of samples. In RPPA technology, the original samples are often diluted successively multiple times, forming dilution series to extend the dynamic range of the measurements and to increase confidence in quantitation. An RPPA experiment is equivalent to running multiple ELISA assays concurrently except that there is usually no known protein concentration from which one can construct a standard response curve. Here, we describe a new method called \u2018serial dilution curve for RPPA data analysis\u2019. Compared with the existing methods, the new method has the advantage of using fewer parameters and offering a simple way of visualizing the raw data. We showed how the method can be used to examine data quality and to obtain robust quantification of protein concentrations.<\/jats:p><jats:p>Availability: A computer program in R for using serial dilution curve for RPPA data analysis is freely available at http:\/\/odin.mdacc.tmc.edu\/~zhangli\/RPPA.<\/jats:p><jats:p>Contact: \u00a0lzhangli@mdanderson.org<\/jats:p>","DOI":"10.1093\/bioinformatics\/btn663","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T01:31:13Z","timestamp":1233192673000},"page":"650-654","source":"Crossref","is-referenced-by-count":47,"title":["Serial dilution curve: a new method for analysis of reverse phase protein array data"],"prefix":"10.1093","volume":"25","author":[{"given":"Li","family":"Zhang","sequence":"first","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyi","family":"Wei","sequence":"additional","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Mao","sequence":"additional","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Liu","sequence":"additional","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"},{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gordon B.","family":"Mills","sequence":"additional","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Coombes","sequence":"additional","affiliation":[{"name":"1 Department of Bioinformatics and Computational Biology, 2Department of Epidemiology, 3Department of Thoracic and Head and Neck Medical Oncology and 4Department of Systems Biology, The University of Texas MD Anderson Cancer Center, 1400 Pressler street, Unit 1410, Houston, TX 77030, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,1,28]]},"reference":[{"key":"2023013110112745500_B1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1038\/ng1987","article-title":"A module of negative feedback regulators defines growth factor signaling","volume":"39","author":"Amit","year":"2007","journal-title":"Nat. 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