{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T05:14:33Z","timestamp":1775538873964,"version":"3.50.1"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"19","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: The rational design of proteins with modified properties, through amino acid substitutions, is of crucial importance in a large variety of applications. Given the huge number of possible substitutions, every protein engineering project would benefit strongly from the guidance of in silico methods able to predict rapidly, and with reasonable accuracy, the stability changes resulting from all possible mutations in a protein.<\/jats:p>\n               <jats:p>Results: We exploit newly developed statistical potentials, based on a formalism that highlights the coupling between four protein sequence and structure descriptors, and take into account the amino acid volume variation upon mutation. The stability change is expressed as a linear combination of these energy functions, whose proportionality coefficients vary with the solvent accessibility of the mutated residue and are identified with the help of a neural network. A correlation coefficient of R = 0.63 and a root mean square error of \u03c3c = 1.15 kcal\/mol between measured and predicted stability changes are obtained upon cross-validation. These scores reach R = 0.79, and \u03c3c = 0.86 kcal\/mol after exclusion of 10% outliers. The predictive power of our method is shown to be significantly higher than that of other programs described in the literature.<\/jats:p>\n               <jats:p>Availability: \u00a0http:\/\/babylone.ulb.ac.be\/popmusic<\/jats:p>\n               <jats:p>Contact: \u00a0ydehouck@ulb.ac.be<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btp445","type":"journal-article","created":{"date-parts":[[2009,8,4]],"date-time":"2009-08-04T20:13:27Z","timestamp":1249416807000},"page":"2537-2543","source":"Crossref","is-referenced-by-count":415,"title":["Fast and accurate predictions of protein stability changes upon mutations using statistical potentials and neural networks: PoPMuSiC-2.0"],"prefix":"10.1093","volume":"25","author":[{"given":"Yves","family":"Dehouck","sequence":"first","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. Roosevelt 50, CP165\/61, 1050 Brussels, Belgium"}]},{"given":"Aline","family":"Grosfils","sequence":"additional","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. Roosevelt 50, CP165\/61, 1050 Brussels, Belgium"}]},{"given":"Benjamin","family":"Folch","sequence":"additional","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. Roosevelt 50, CP165\/61, 1050 Brussels, Belgium"}]},{"given":"Dimitri","family":"Gilis","sequence":"additional","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. Roosevelt 50, CP165\/61, 1050 Brussels, Belgium"}]},{"given":"Philippe","family":"Bogaerts","sequence":"additional","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. Roosevelt 50, CP165\/61, 1050 Brussels, Belgium"}]},{"given":"Marianne","family":"Rooman","sequence":"additional","affiliation":[{"name":"1 Bioinformatique g\u00e9nomique et structurale and 2Mod\u00e9lisation et contr\u00f4le de bioproc\u00e9d\u00e9s, Universit\u00e9 Libre de Bruxelles. Av Fr. 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