{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T16:41:38Z","timestamp":1739378498823,"version":"3.37.0"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: The Exon Array Analyzer (EAA) is a web server, which provides a user-friendly interface to identify alternative splicing events analyzed with Affymetrix Exon Arrays. The EAA implements the Splice Index algorithm to identify differential expressed exons. The use of various filters allows reduction of the number of false positive hits. Results are presented with detailed annotation information and graphics to identify splice events and to facilitate biological validations. To demonstrate the versatility of the EAA, we analyzed exon arrays of 11 different murine tissues using sample data provided by Affymetrix (http:\/\/www.affymetrix.com). Data from the heart were compared with other tissues to identify exons that undergo heart-specific alternatively splicing, resulting in the identification of 885 differentially expressed probe sets in 649 genes.<\/jats:p><jats:p>Availability: The web interface is available at http:\/\/EAA.mpi-bn.mpg.de\/. Detailed documentation is available on the EAA web site (http:\/\/EAA.mpi-bn.mpg.de\/supp.php) including screen shots, example analyzes and step by step instructions.<\/jats:p><jats:p>Contact: \u00a0thomas.braun@mpi-bn.mpg.de<\/jats:p><jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btp577","type":"journal-article","created":{"date-parts":[[2009,10,7]],"date-time":"2009-10-07T03:44:47Z","timestamp":1254887087000},"page":"3323-3324","source":"Crossref","is-referenced-by-count":16,"title":["Exon Array Analyzer: a web interface for Affymetrix exon array analysis"],"prefix":"10.1093","volume":"25","author":[{"given":"Pascal","family":"Gellert","sequence":"first","affiliation":[{"name":"Max-Planck-Institute for Heart and Lung Research, Parkstrasse 1, 61231 Bad Nauheim, Germany"}]},{"given":"Shizuka","family":"Uchida","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute for Heart and Lung Research, Parkstrasse 1, 61231 Bad Nauheim, Germany"}]},{"given":"Thomas","family":"Braun","sequence":"additional","affiliation":[{"name":"Max-Planck-Institute for Heart and Lung Research, Parkstrasse 1, 61231 Bad Nauheim, Germany"}]}],"member":"286","published-online":{"date-parts":[[2009,10,12]]},"reference":[{"year":"2005","author":"Affymetrix Inc","journal-title":"Guide to probe logarithmic intensity error (PLIER) estimation.","key":"2023013112143864100_B1"},{"key":"2023013112143864100_B2","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","article-title":"Controlling the false discovery rate: a practical and powerful approach to multiple testing","volume":"57","author":"Benjamini","year":"1995","journal-title":"J. R. Stat. Soc. Ser. B (Methodol.)"},{"key":"2023013112143864100_B3","doi-asserted-by":"crossref","first-page":"907","DOI":"10.1126\/science.1069415","article-title":"Genomewide analysis of mRNA processing in yeast using Splicing-Specific microarrays","volume":"296","author":"Clark","year":"2002","journal-title":"Science"},{"key":"2023013112143864100_B4","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1093\/biostatistics\/4.2.249","article-title":"Exploration, normalization, and summaries of high density oligonucleotide array probe level data","volume":"4","author":"Irizarry","year":"2003","journal-title":"Biostat."},{"key":"2023013112143864100_B5","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1126\/science.1090100","article-title":"Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays","volume":"302","author":"Johnson","year":"2003","journal-title":"Science"},{"key":"2023013112143864100_B6","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1186\/1471-2164-10-100","article-title":"An integrated approach for the systematic identification and characterization of heart-enriched genes with unknown functions","volume":"10","author":"Uchida","year":"2009","journal-title":"BMC Genomics"},{"key":"2023013112143864100_B7","doi-asserted-by":"crossref","first-page":"749","DOI":"10.1038\/nrg2164","article-title":"Splicing in disease: disruption of the splicing code and the decoding machinery","volume":"8","author":"Wang","year":"2007","journal-title":"Nat. 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Genet."}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/25\/24\/3323\/48997291\/bioinformatics_25_24_3323.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/25\/24\/3323\/48997291\/bioinformatics_25_24_3323.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T16:04:55Z","timestamp":1739376295000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/25\/24\/3323\/235095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,10,12]]},"references-count":7,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2009,12,15]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btp577","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"type":"electronic","value":"1367-4811"},{"type":"print","value":"1367-4803"}],"subject":[],"published-other":{"date-parts":[[2009,12,15]]},"published":{"date-parts":[[2009,10,12]]}}}