{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T14:02:07Z","timestamp":1761919327971},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"14","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Molecular chaperones prevent the aggregation of their substrate proteins and thereby ensure that they reach their functional native state. The bacterial GroEL\/ES chaperonin system is understood in great detail on a structural, mechanistic and functional level; its interactors in Escherichia coli have been identified and characterized. However, a long-standing question in the field is: What makes a protein a chaperone substrate?<\/jats:p>\n               <jats:p>Results: Here we identify, using a bioinformatics-based approach a simple set of quantities, which characterize the GroEL-substrate proteome. We define three novel parameters differentiating GroEL interactors from other cellular proteins: lower rate of evolution, hydrophobicity and aggregation propensity. Combining them with other known features to a simple Bayesian predictor allows us to identify known homologous and heterologous GroEL substrateproteins. We discuss our findings in relation to established mechanisms of protein folding and evolutionary buffering by chaperones.<\/jats:p>\n               <jats:p>Contact: \u00a0tobias.maier@crg.es<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data is available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btq287","type":"journal-article","created":{"date-parts":[[2010,6,3]],"date-time":"2010-06-03T01:10:05Z","timestamp":1275527405000},"page":"1685-1689","source":"Crossref","is-referenced-by-count":24,"title":["A more precise characterization of chaperonin substrates"],"prefix":"10.1093","volume":"26","author":[{"given":"Emanuele","family":"Raineri","sequence":"first","affiliation":[{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"},{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Ribeca","sequence":"additional","affiliation":[{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"},{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Serrano","sequence":"additional","affiliation":[{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Maier","sequence":"additional","affiliation":[{"name":"1 CNAG Centro Nacional de An\u00e1lisis Gen\u00f3mico, Parc Cientific de Barcelona, Baldiri Reixac 4, E-08028 Barcelona, 2Center for Genomic Regulation, Bioinformatics and Genomics Unit and 3Center for Genomic Regulation, Systems Biology Unit, Calle Dr. Aiguader 88, E-08003 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,6,2]]},"reference":[{"key":"2023012507574698800_B1","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1098\/rsif.2008.0185","article-title":"Development of free-energy-based models for chaperonin containing TCP-1 mediated folding of actin","volume":"5","author":"Altschuler","year":"2008","journal-title":"J. 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