{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T07:05:35Z","timestamp":1771484735497,"version":"3.50.1"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"16","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":2253,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: Huge amount of metagenomic sequence data have been produced as a result of the rapidly increasing efforts worldwide in studying microbial communities as a whole. Most, if not all, sequenced metagenomes are complex mixtures of chromosomal and plasmid sequence fragments from multiple organisms, possibly from different kingdoms. Computational methods for prediction of genomic elements such as genes are significantly different for chromosomes and plasmids, hence raising the need for separation of chromosomal from plasmid sequences in a metagenome. We present a program for classification of a metagenome set into chromosomal and plasmid sequences, based on their distinguishing pentamer frequencies. On a large training set consisting of all the sequenced prokaryotic chromosomes and plasmids, the program achieves \u223c92% in classification accuracy. On a large set of simulated metagenomes with sequence lengths ranging from 300 bp to 100 kbp, the program has classification accuracy from 64.45% to 88.75%. On a large independent test set, the program achieves 88.29% classification accuracy.<\/jats:p>\n               <jats:p>Availability: The program has been implemented as a standalone prediction program, cBar, which is available at http:\/\/csbl.bmb.uga.edu\/\u223cffzhou\/cBar<\/jats:p>\n               <jats:p>Contact: \u00a0xyn@bmb.uga.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btq299","type":"journal-article","created":{"date-parts":[[2010,6,11]],"date-time":"2010-06-11T00:51:37Z","timestamp":1276217497000},"page":"2051-2052","source":"Crossref","is-referenced-by-count":106,"title":["cBar: a computer program to distinguish plasmid-derived from chromosome-derived sequence fragments in metagenomics data"],"prefix":"10.1093","volume":"26","author":[{"given":"Fengfeng","family":"Zhou","sequence":"first","affiliation":[{"name":"1 Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology, and Institute of Bioinformatics, and BioEnergy Science Center (BESC), University of Georgia, Athens, GA 30602, USA and 2College of Computer Science and Technology, Jilin University, Changchun, Jilin 130012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[{"name":"1 Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology, and Institute of Bioinformatics, and BioEnergy Science Center (BESC), University of Georgia, Athens, GA 30602, USA and 2College of Computer Science and Technology, Jilin University, Changchun, Jilin 130012, China"},{"name":"1 Computational Systems Biology Laboratory, Department of Biochemistry and Molecular Biology, and Institute of Bioinformatics, and BioEnergy Science Center (BESC), University of Georgia, Athens, GA 30602, USA and 2College of Computer Science and Technology, Jilin University, Changchun, Jilin 130012, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,8,2]]},"reference":[{"key":"2023012508025292300_B1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.2307\/2347628","article-title":"Ridge estimators in logistic regression","volume":"41","author":"Cessie","year":"1992","journal-title":"Appl. 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