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We show an example of its application in our laboratory to find sequences in Brachiaria with similarity to ESTs related to apomixis.<\/jats:p>\n               <jats:p>Contact: \u00a0srestrep@uniandes.edu.co; j.tohme@cgiar.org<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btq312","type":"journal-article","created":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T03:13:23Z","timestamp":1276571603000},"page":"2053-2054","source":"Crossref","is-referenced-by-count":6,"title":["A new method for designing degenerate primers and its use in the identification of sequences in <i>Brachiaria<\/i> showing similarity to apomixis-associated genes"],"prefix":"10.1093","volume":"26","author":[{"given":"Eduardo","family":"Gorr\u00f3n","sequence":"first","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"},{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Fausto","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Diana","family":"Bernal","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Luis Miguel","family":"Rodriguez-Rojas","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Adriana","family":"Bernal","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Silvia","family":"Restrepo","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]},{"given":"Joe","family":"Tohme","sequence":"additional","affiliation":[{"name":"1 Centro Internacional de Agricultura Tropical (CIAT), A.A. 6713, Cali and 2Laboratorio de Micolog\u00eda y Fitopatolog\u00eda, Universidad de los Andes, Carrera 1 No 18A-10, Bogot\u00e1, Colombia"}]}],"member":"286","published-online":{"date-parts":[[2010,6,14]]},"reference":[{"key":"2023012508032192000_B1","doi-asserted-by":"crossref","first-page":"879","DOI":"10.1007\/s11103-004-5211-y","article-title":"Isolation of candidate genes for apomixis in Poa pratensis L","volume":"56","author":"Albertini","year":"2004","journal-title":"Plant Mol. 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