{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T17:07:24Z","timestamp":1761844044626},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Immunoglobulin heavy chain genes are formed by recombination of genes randomly selected from sets of IGHV, IGHD and IGHJ genes. Utilities have been developed to identify genes that contribute to observed VDJ rearrangements, but in the absence of datasets of known rearrangements, the evaluation of these utilities is problematic. We have analyzed thousands of VDJ rearrangements from an individual (S22) whose IGHV, IGHD and IGHJ genotype can be inferred from the dataset. Knowledge of this genotype means that the Stanford_S22 dataset can serve to benchmark the performance of IGH alignment utilities.<\/jats:p>\n               <jats:p>Results: We evaluated the performance of seven utilities. Failure to partition a sequence into genes present in the S22 genome was considered an error, and error rates for different utilities ranged from 7.1% to 13.7%.<\/jats:p>\n               <jats:p>Availability: \u00a0Supplementary data includes the S22 genotypes and alignments. The Stanford_S22 dataset and an evaluation tool is available at http:\/\/www.emi.unsw.edu.au\/~ihmmune\/IGHUtilityEval\/.<\/jats:p>\n               <jats:p>Contact: \u00a0katherine.jackson@unsw.edu.au<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btq604","type":"journal-article","created":{"date-parts":[[2010,10,30]],"date-time":"2010-10-30T00:45:14Z","timestamp":1288399514000},"page":"3129-3130","source":"Crossref","is-referenced-by-count":20,"title":["Benchmarking the performance of human antibody gene alignment utilities using a 454 sequence dataset"],"prefix":"10.1093","volume":"26","author":[{"given":"Katherine J. L.","family":"Jackson","sequence":"first","affiliation":[{"name":"1 School of Computer Science and Engineering, 2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia and 3Department of Pathology, Stanford University, Stanford, CA 94305, USA"},{"name":"1 School of Computer Science and Engineering, 2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia and 3Department of Pathology, Stanford University, Stanford, CA 94305, USA"}]},{"given":"Scott","family":"Boyd","sequence":"additional","affiliation":[{"name":"1 School of Computer Science and Engineering, 2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia and 3Department of Pathology, Stanford University, Stanford, CA 94305, USA"}]},{"given":"Bruno A.","family":"Ga\u00ebta","sequence":"additional","affiliation":[{"name":"1 School of Computer Science and Engineering, 2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia and 3Department of Pathology, Stanford University, Stanford, CA 94305, USA"}]},{"given":"Andrew M.","family":"Collins","sequence":"additional","affiliation":[{"name":"1 School of Computer Science and Engineering, 2School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia and 3Department of Pathology, Stanford University, Stanford, CA 94305, USA"}]}],"member":"286","published-online":{"date-parts":[[2010,10,29]]},"reference":[{"key":"2023012508032388900_B1","doi-asserted-by":"crossref","first-page":"6986","DOI":"10.4049\/jimmunol.1000445","article-title":"Individual variation in the germline immunoglobulin gene repertoire inferred from VDJ rearrangements","volume":"184","author":"Boyd","year":"2010","journal-title":"J. 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