{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T19:20:05Z","timestamp":1771701605988,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Immunoassays are primary diagnostic and research tools throughout the medical and life sciences. The common approach to the processing of immunoassay data involves estimation of the calibration curve followed by inversion of the calibration function to read off the concentration estimates. This approach, however, does not lend itself easily to acceptable estimation of confidence limits on the estimated concentrations. Such estimates must account for uncertainty in the calibration curve as well as uncertainty in the target measurement. Even point estimates can be problematic: because of the non-linearity of calibration curves and error heteroscedasticity, the neglect of components of measurement error can produce significant bias.<\/jats:p>\n               <jats:p>Methods: We have developed a Bayesian approach for the estimation of concentrations from immunoassay data that treats the propagation of measurement error appropriately. The method uses Markov Chain Monte Carlo (MCMC) to approximate the posterior distribution of the target concentrations and numerically compute the relevant summary statistics. Software implementing the method is freely available for public use.<\/jats:p>\n               <jats:p>Results: The new method was tested on both simulated and experimental datasets with different measurement error models. The method outperformed the common inverse method on samples with large measurement errors. Even in cases with extreme measurements where the common inverse method failed, our approach always generated reasonable estimates for the target concentrations.<\/jats:p>\n               <jats:p>Availability: Project name: Baecs; Project home page: www.computationalimmunology.org\/utilities\/; Operating systems: Linux, MacOS X and Windows; Programming language: C++; License: Free for Academic Use.<\/jats:p>\n               <jats:p>Contact: \u00a0feng.feng@duke.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btq686","type":"journal-article","created":{"date-parts":[[2010,12,14]],"date-time":"2010-12-14T04:21:31Z","timestamp":1292300491000},"page":"707-712","source":"Crossref","is-referenced-by-count":17,"title":["A Bayesian approach for estimating calibration curves and unknown concentrations in immunoassays"],"prefix":"10.1093","volume":"27","author":[{"given":"Feng","family":"Feng","sequence":"first","affiliation":[{"name":"1 Center for Computational Immunology, Department of Biostatistics and Bioinformatics, Duke University Medical Center, 2424 Erwin Road, Hock Plaza Suite 1102, 2Department of Immunology and 3Department of Statistical Science, Duke University, Durham, NC 27705, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Sales","sequence":"additional","affiliation":[{"name":"1 Center for Computational Immunology, Department of Biostatistics and Bioinformatics, Duke University Medical Center, 2424 Erwin Road, Hock Plaza Suite 1102, 2Department of Immunology and 3Department of Statistical Science, Duke University, Durham, NC 27705, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.","family":"Kepler","sequence":"additional","affiliation":[{"name":"1 Center for Computational Immunology, Department of Biostatistics and Bioinformatics, Duke University Medical Center, 2424 Erwin Road, Hock Plaza Suite 1102, 2Department of Immunology and 3Department of Statistical Science, Duke University, Durham, NC 27705, USA"},{"name":"1 Center for Computational Immunology, Department of Biostatistics and Bioinformatics, Duke University Medical Center, 2424 Erwin Road, Hock Plaza Suite 1102, 2Department of Immunology and 3Department of Statistical Science, Duke University, Durham, NC 27705, USA"},{"name":"1 Center for Computational Immunology, Department of Biostatistics and Bioinformatics, Duke University Medical Center, 2424 Erwin Road, Hock Plaza Suite 1102, 2Department of Immunology and 3Department of Statistical Science, Duke University, Durham, NC 27705, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,12,12]]},"reference":[{"key":"2023012511573059100_B1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/S0300-483X(98)00063-8","article-title":"Cytokine assays in human sera and tissues","volume":"129","author":"Bienvenu","year":"1998","journal-title":"Toxicology"},{"key":"2023012511573059100_B2","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1198\/000313008X366983","article-title":"Parametric nonparameteric statistics","volume":"62","author":"Christensen","year":"2008","journal-title":"Am. 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