{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:30:51Z","timestamp":1759336251642},"reference-count":6,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":2055,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: The SFF file format produced by Roche's 454 sequencing technology is a compact, binary format that contains the flow values that are used for base and quality calling of the reads. Applications, e.g. in metagenomics, often depend on accurate sequence information, and access to flow values is important to estimate the probability of errors. Unfortunately, the programs supplied by Roche for accessing this information are not publicly available. Flower is a program that can extract the information contained in SFF files, and convert it to various textual output formats.<\/jats:p>\n               <jats:p>Availability: Flower is freely available under the General Public License.<\/jats:p>\n               <jats:p>Contact: \u00a0ketil.malde@imr.no<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr063","type":"journal-article","created":{"date-parts":[[2011,2,18]],"date-time":"2011-02-18T01:18:15Z","timestamp":1297991895000},"page":"1041-1042","source":"Crossref","is-referenced-by-count":5,"title":["Flower: extracting information from pyrosequencing data"],"prefix":"10.1093","volume":"27","author":[{"given":"Ketil","family":"Malde","sequence":"first","affiliation":[{"name":"The Norwegian Marine Data Centre, Institute of Marine Research, Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,2,16]]},"reference":[{"key":"2023012512164025000_B1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"2023012512164025000_B2","article-title":"Genome sequence assembly using trace signals and additional sequence information","author":"Chevreux","year":"1999","journal-title":"German Conference on Bioinformatics."},{"key":"2023012512164025000_B3","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1093\/nar\/gkp1137","article-title":"The Sanger FASTQ file format for sequences with quality scores, and the Solexa\/Illumina FASTQ variants","volume":"38","author":"Cock","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"2023012512164025000_B4","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1038\/nature03959","article-title":"Genome sequencing in microfabricated high-density picolitre reactors","volume":"437","author":"Margulies","year":"2005","journal-title":"Nature"},{"key":"2023012512164025000_B5","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1038\/nmeth.1361","article-title":"Accurate determination of microbial diversity from 454 pyrosequencing data","volume":"6","author":"Quince","year":"2009","journal-title":"Nat. Methods"},{"key":"2023012512164025000_B6","author":"Roche","year":"2009","journal-title":"Genome Sequencer FLX System Software Manual, version 2.3, General Overview and File Formats"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/27\/7\/1041\/48868382\/bioinformatics_27_7_1041.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/27\/7\/1041\/48868382\/bioinformatics_27_7_1041.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T14:40:05Z","timestamp":1674657605000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/27\/7\/1041\/232549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,16]]},"references-count":6,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,4,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btr063","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2011,4,1]]},"published":{"date-parts":[[2011,2,16]]}}}