{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T05:11:32Z","timestamp":1781154692331,"version":"3.54.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: uMeltSM is a flexible web-based tool for predicting DNA melting curves and denaturation profiles of PCR products. The user defines an amplicon sequence and chooses a set of thermodynamic and experimental parameters that include nearest neighbor stacking energies, loop entropy effects, cation (monovalent and Mg++) concentrations and a temperature range. Using an accelerated partition function algorithm along with chosen parameter values, uMelt interactively calculates and visualizes the mean helicity and the dissociation probability at each sequence position at temperatures within the temperature range. Predicted curves display the mean helicity as a function of temperature or as derivative plots. Predicted profiles display stability as a function of sequence position either as 50% helicity temperatures or as the helicity probability at specific temperatures. The loss of helicity associated with increasing temperature may be viewed dynamically to visualize domain formation within the molecule. Results from fluorescent high-resolution melting experiments match the number of predicted melting domains and their relative temperatures. However, the absolute melting temperatures vary with the selected thermodynamic parameters and current libraries do not account for the rapid melting rates and helix stabilizing dyes used in fluorescent melting experiments. uMelt provides a convenient platform for simulation and design of high-resolution melting assays.<\/jats:p>\n               <jats:p>Availability and implementation: The application was developed in Actionscript and can be found online at http:\/\/www.dna.utah.edu\/umelt\/umelt.html. Adobe Flash is required to run in all browsers.<\/jats:p>\n               <jats:p>Contact: \u00a0zach.dwight@path.utah.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr065","type":"journal-article","created":{"date-parts":[[2011,2,8]],"date-time":"2011-02-08T05:06:38Z","timestamp":1297141598000},"page":"1019-1020","source":"Crossref","is-referenced-by-count":237,"title":["uMELT: prediction of high-resolution melting curves and dynamic melting profiles of PCR products in a rich web application"],"prefix":"10.1093","volume":"27","author":[{"given":"Zachary","family":"Dwight","sequence":"first","affiliation":[{"name":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Palais","sequence":"additional","affiliation":[{"name":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl T.","family":"Wittwer","sequence":"additional","affiliation":[{"name":"1 School of Computing, University of Utah, 50 South Central Campus Drive, Room 3190, 2Department of Mathematics, University of Utah, 155 South 1400 East, Room 233 and 3Department of Pathology, University of Utah, 15 North Medical Drive East, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,2,7]]},"reference":[{"key":"2023012512162586600_B1","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1093\/bioinformatics\/15.5.370","article-title":"Statistical mechanical simulation of polymeric DNA melting with MELTSIM","volume":"15","author":"Blake","year":"1999","journal-title":"Bioinformatics"},{"key":"2023012512162586600_B2","doi-asserted-by":"crossref","first-page":"3323","DOI":"10.1093\/nar\/26.14.3323","article-title":"Thermal stability of DNA","volume":"26","author":"Blake","year":"1998","journal-title":"Nucleic Acids Res."},{"key":"2023012512162586600_B3","doi-asserted-by":"crossref","first-page":"061911","DOI":"10.1103\/PhysRevE.68.061911","article-title":"Reparametrizing the loop entropy weights: effect on DNA melting curves","volume":"68","author":"Blossey","year":"2003","journal-title":"Phys. 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