{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:15:56Z","timestamp":1783098956813,"version":"3.54.6"},"reference-count":12,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":2048,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.<\/jats:p>\n               <jats:p>Results: PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.<\/jats:p>\n               <jats:p>Availability: PrimerProspector is open-source software available at http:\/\/pprospector.sourceforge.net<\/jats:p>\n               <jats:p>Contact: \u00a0rob.knight@colorado.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr087","type":"journal-article","created":{"date-parts":[[2011,2,25]],"date-time":"2011-02-25T01:38:20Z","timestamp":1298597900000},"page":"1159-1161","source":"Crossref","is-referenced-by-count":347,"title":["PrimerProspector: <i>de novo<\/i> design and taxonomic analysis of barcoded polymerase chain reaction primers"],"prefix":"10.1093","volume":"27","author":[{"given":"William A.","family":"Walters","sequence":"first","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Gregory","family":"Caporaso","sequence":"additional","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian L.","family":"Lauber","sequence":"additional","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Berg-Lyons","sequence":"additional","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Fierer","sequence":"additional","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"},{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob","family":"Knight","sequence":"additional","affiliation":[{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"},{"name":"1 Department of Molecular, Cellular, and Developmental Biology, 2Department of Chemistry and Biochemistry, 3Cooperative Institute for Research in Environmental Sciences, 4Department of Ecology and Evolutionary Biology, University of Colorado at Boulder, Boulder, CO 80309, USA and 5Howard Hughes Medical Institute, Boulder, CO, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,2,23]]},"reference":[{"key":"2023061311474422200_B1","doi-asserted-by":"crossref","first-page":"3481","DOI":"10.1093\/nar\/gkf450","article-title":"PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database","volume":"30","author":"Ashelford","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"2023061311474422200_B2","article-title":"Examining the global distribution of dominant archaeal populations in soil","author":"Bates","year":"2010","journal-title":"ISME J."},{"key":"2023061311474422200_B3","article-title":"Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample","author":"Caporaso","year":"2010","journal-title":"Proc. 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