{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:20:41Z","timestamp":1767907241297,"version":"3.49.0"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"13","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":1937,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/2.5"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: 454 pyrosequencing, by Roche Diagnostics, has emerged as an alternative to Sanger sequencing when it comes to read lengths, performance and cost, but shows higher per-base error rates. Although there are several tools available for noise removal, targeting different application fields, data interpretation would benefit from a better understanding of the different error types.<\/jats:p>\n               <jats:p>Results: By exploring 454 raw data, we quantify to what extent different factors account for sequencing errors. In addition to the well-known homopolymer length inaccuracies, we have identified errors likely to originate from other stages of the sequencing process. We use our findings to extend the flowsim pipeline with functionalities to simulate these errors, and thus enable a more realistic simulation of 454 pyrosequencing data with flowsim.<\/jats:p>\n               <jats:p>Availability: The flowsim pipeline is freely available under the General Public License from http:\/\/biohaskell.org\/Applications\/FlowSim.<\/jats:p>\n               <jats:p>Contact: \u00a0susanne.balzer@imr.no<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr251","type":"journal-article","created":{"date-parts":[[2011,6,17]],"date-time":"2011-06-17T23:32:32Z","timestamp":1308353552000},"page":"i304-i309","source":"Crossref","is-referenced-by-count":71,"title":["Systematic exploration of error sources in pyrosequencing flowgram data"],"prefix":"10.1093","volume":"27","author":[{"given":"Susanne","family":"Balzer","sequence":"first","affiliation":[{"name":"1 Institute of Marine Research, P.O. Box 1870, N\u22125817 Bergen, 2Department of Informatics, University of Bergen, P.O. Box 7803, N-5020 Bergen and 3Computational Biology Unit, Uni Computing, Thorm\u00f8hlensgate 55, N-5008 Bergen, Norway"},{"name":"1 Institute of Marine Research, P.O. Box 1870, N\u22125817 Bergen, 2Department of Informatics, University of Bergen, P.O. Box 7803, N-5020 Bergen and 3Computational Biology Unit, Uni Computing, Thorm\u00f8hlensgate 55, N-5008 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ketil","family":"Malde","sequence":"additional","affiliation":[{"name":"1 Institute of Marine Research, P.O. Box 1870, N\u22125817 Bergen, 2Department of Informatics, University of Bergen, P.O. Box 7803, N-5020 Bergen and 3Computational Biology Unit, Uni Computing, Thorm\u00f8hlensgate 55, N-5008 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inge","family":"Jonassen","sequence":"additional","affiliation":[{"name":"1 Institute of Marine Research, P.O. Box 1870, N\u22125817 Bergen, 2Department of Informatics, University of Bergen, P.O. Box 7803, N-5020 Bergen and 3Computational Biology Unit, Uni Computing, Thorm\u00f8hlensgate 55, N-5008 Bergen, Norway"},{"name":"1 Institute of Marine Research, P.O. Box 1870, N\u22125817 Bergen, 2Department of Informatics, University of Bergen, P.O. Box 7803, N-5020 Bergen and 3Computational Biology Unit, Uni Computing, Thorm\u00f8hlensgate 55, N-5008 Bergen, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,6,14]]},"reference":[{"key":"2023012512144887700_B1","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. Mol. 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