{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,14]],"date-time":"2026-06-14T08:49:25Z","timestamp":1781426965734,"version":"3.54.1"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Motivation: A variety of pocket detection algorithms are now freely or commercially available to the scientific community for the analysis of static protein structures. However, since proteins are dynamic entities, enhancing the capabilities of these programs for the straightforward detection and characterization of cavities taking into account protein conformational ensembles should be valuable for capturing the plasticity of pockets, and therefore allow gaining insight into structure\u2013function relationships.<\/jats:p><jats:p>Results: This article describes a new method, called MDpocket, providing a fast, free and open-source tool for tracking small molecule binding sites and gas migration pathways on molecular dynamics (MDs) trajectories or other conformational ensembles. MDpocket is based on the fpocket cavity detection algorithm and a valuable contribution to existing analysis tools. The capabilities of MDpocket are illustrated for three relevant cases: (i) the detection of transient subpockets using an ensemble of crystal structures of HSP90; (ii) the detection of known xenon binding sites and migration pathways in myoglobin; and (iii) the identification of suitable pockets for molecular docking in P38 Map kinase.<\/jats:p><jats:p>Availability: MDpocket is free and open-source software and can be downloaded at http:\/\/fpocket.sourceforge.net.<\/jats:p><jats:p>Contact: \u00a0pschmidtke@ub.edu<\/jats:p><jats:p>Supplementary Information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr550","type":"journal-article","created":{"date-parts":[[2011,10,4]],"date-time":"2011-10-04T03:14:50Z","timestamp":1317698090000},"page":"3276-3285","source":"Crossref","is-referenced-by-count":369,"title":["MDpocket: open-source cavity detection and characterization on molecular dynamics trajectories"],"prefix":"10.1093","volume":"27","author":[{"given":"Peter","family":"Schmidtke","sequence":"first","affiliation":[{"name":"1 Departament de Fisicoqu\u00edmica and Institut de Biomedicina (IBUB), Facultat de Farm\u00e0cia, Universitat de Barcelona, 08028 Barcelona, Spain, 2Equipe de Dynamique des Assemblages Membranaires, UMR No. 7565, Centre National de la Recherche Scientifique-Universit\u00e9 Henri Poincar\u00e9, Nancy, France and 3Instituci\u00f3 Catalana de Recerca i Estudis Avanats (ICREA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Bidon-Chanal","sequence":"additional","affiliation":[{"name":"1 Departament de Fisicoqu\u00edmica and Institut de Biomedicina (IBUB), Facultat de Farm\u00e0cia, Universitat de Barcelona, 08028 Barcelona, Spain, 2Equipe de Dynamique des Assemblages Membranaires, UMR No. 7565, Centre National de la Recherche Scientifique-Universit\u00e9 Henri Poincar\u00e9, Nancy, France and 3Instituci\u00f3 Catalana de Recerca i Estudis Avanats (ICREA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. Javier","family":"Luque","sequence":"additional","affiliation":[{"name":"1 Departament de Fisicoqu\u00edmica and Institut de Biomedicina (IBUB), Facultat de Farm\u00e0cia, Universitat de Barcelona, 08028 Barcelona, Spain, 2Equipe de Dynamique des Assemblages Membranaires, UMR No. 7565, Centre National de la Recherche Scientifique-Universit\u00e9 Henri Poincar\u00e9, Nancy, France and 3Instituci\u00f3 Catalana de Recerca i Estudis Avanats (ICREA)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Barril","sequence":"additional","affiliation":[{"name":"1 Departament de Fisicoqu\u00edmica and Institut de Biomedicina (IBUB), Facultat de Farm\u00e0cia, Universitat de Barcelona, 08028 Barcelona, Spain, 2Equipe de Dynamique des Assemblages Membranaires, UMR No. 7565, Centre National de la Recherche Scientifique-Universit\u00e9 Henri Poincar\u00e9, Nancy, France and 3Instituci\u00f3 Catalana de Recerca i Estudis Avanats (ICREA)"},{"name":"1 Departament de Fisicoqu\u00edmica and Institut de Biomedicina (IBUB), Facultat de Farm\u00e0cia, Universitat de Barcelona, 08028 Barcelona, Spain, 2Equipe de Dynamique des Assemblages Membranaires, UMR No. 7565, Centre National de la Recherche Scientifique-Universit\u00e9 Henri Poincar\u00e9, Nancy, France and 3Instituci\u00f3 Catalana de Recerca i Estudis Avanats (ICREA)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,10,3]]},"reference":[{"key":"2023012511035184600_B1","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1038\/nature04716","article-title":"Crystal structure of an Hsp90-nucleotide-p23\/Sba1 closed chaperone complex","volume":"440","author":"Ali","year":"2006","journal-title":"Nature"},{"key":"2023012511035184600_B2","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1074\/mcp.M400159-MCP200","article-title":"Pocketome via comprehensive identification and classification of ligand binding envelopes","volume":"4","author":"An","year":"2005","journal-title":"Mol. 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