{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T19:12:52Z","timestamp":1674846772676},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure and JavaScript to interact with the users. In VizPrimer, the users can focus their attention on the gene structure and primer design strategy, without wasting time calculating the exon positions of splice variants or manually configuring complicated parameters. In addition, VizPrimer is also suitable for the design of PCR primers for amplifying open reading frames and detecting single nucleotide polymorphisms (SNPs).<\/jats:p>\n               <jats:p>Availability: VizPrimer is freely available at http:\/\/biocompute.bmi.ac.cn\/CZlab\/VizPrimer\/. The web server supported browsers: Chrome (\u22655.0), Firefox (\u22653.0), Safari (\u22654.0) and Opera (\u226510.0).<\/jats:p>\n               <jats:p>Contact: \u00a0zhangcg@bmi.ac.cn; yangyi528@vip.sina.com<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr582","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T02:07:11Z","timestamp":1319162831000},"page":"3432-3434","source":"Crossref","is-referenced-by-count":6,"title":["VizPrimer: a web server for visualized PCR primer design based on known gene structure"],"prefix":"10.1093","volume":"27","author":[{"given":"Yang","family":"Zhou","sequence":"first","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"},{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wubin","family":"Qu","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Lu","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolei","family":"Wang","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongsheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenggang","family":"Zhang","sequence":"additional","affiliation":[{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, China"},{"name":"1 Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, Cognitive and Mental Health Research Center, Beijing 100850, 2College of Life Science, Sichuan University, Key Laboratory of Bio-Resources and Eco-Environment of MOE, Chengdu 610064, 3Beijing Institute of Health Service and Medical Information, Beijing 100850 and 4School of Life Science, Anhui Medical University, Anhui 230032, 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