{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:43:12Z","timestamp":1790487792123,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Multi-view microscopy techniques such as Light-Sheet Fluorescence Microscopy (LSFM) are powerful tools for 3D + time studies of live embryos in developmental biology. The sample is imaged from several points of view, acquiring a set of 3D views that are then combined or fused in order to overcome their individual limitations. Views fusion is still an open problem despite recent contributions in the field.<\/jats:p>\n               <jats:p>Results: We developed a wavelet-based multi-view fusion method that, due to wavelet decomposition properties, is able to combine the complementary directional information from all available views into a single volume. Our method is demonstrated on LSFM acquisitions from live sea urchin and zebrafish embryos. The fusion results show improved overall contrast and details when compared with any of the acquired volumes. The proposed method does not need knowledge of the system's point spread function (PSF) and performs better than other existing PSF independent fusion methods.<\/jats:p>\n               <jats:p>Availability and Implementation: The described method was implemented in Matlab (The Mathworks, Inc., USA) and a graphic user interface was developed in Java.<\/jats:p>\n               <jats:p>The software, together with two sample datasets, is available at http:\/\/www.die.upm.es\/im\/software\/SPIMFusionGUI.zip<\/jats:p>\n               <jats:p>A public release, free of charge for non-commercial use, is planned after the publication of this article.<\/jats:p>\n               <jats:p>Contact: \u00a0jlrubio@die.upm.es; nadine.peyrieras@inaf.cnrs-gif.fr; mledesma@die.upm.es<\/jats:p>\n               <jats:p>Supplementary Information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr609","type":"journal-article","created":{"date-parts":[[2011,11,10]],"date-time":"2011-11-10T06:32:04Z","timestamp":1320906724000},"page":"238-245","source":"Crossref","is-referenced-by-count":35,"title":["Wavelet-based image fusion in multi-view three-dimensional microscopy"],"prefix":"10.1093","volume":"28","author":[{"given":"Jose L.","family":"Rubio-Guivernau","sequence":"first","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"},{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasily","family":"Gurchenkov","sequence":"additional","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel A.","family":"Luengo-Oroz","sequence":"additional","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"},{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, 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Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Santos","sequence":"additional","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"},{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Peyrieras","sequence":"additional","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad Polit\u00e9nica de Madrid, Madrid, 2Centro de Investigaci\u00f3n Biom\u00e9dica en Red en Bioingenier\u00eda, Biomateriales y Nanomedicina (CIBER-BBN), Spain, 3Institut des Syst\u00e8mes complexes & NeD, Institut de Neurobiologie Alfred Fessard, CNRS, Gif-sur-Yvette and 4Institut des Syst\u00e8mes complexes & CREA, \u00c9cole Polytechnique, CNRS, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Ledesma-Carbayo","sequence":"additional","affiliation":[{"name":"1 Biomedical Image Technologies, ETSI Telecomunicaci\u00f3n, Universidad 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