{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T22:31:07Z","timestamp":1778884267259,"version":"3.51.4"},"reference-count":7,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: Differential scanning fluorimetry (DSF) is a rapid technique that can be used in structural biology to study protein\u2013ligand interactions. We have developed DMAN, a novel tool to analyse multi-well plate data obtained in DSF experiments. DMAN is easy to install and provides a user-friendly interface. Multi-well plate layouts can be designed by the user and experimental data can be annotated and analysed by DMAN according to the specified plate layout. Statistical tests for significance are performed automatically, and graphical tools are also provided to assist in data analysis. The modular concept of this software will allow easy development of other multi-well plate analysis applications in the future.<\/jats:p>\n               <jats:p>Availability and implementation: DMAN is implemented in Java to provide a cross-platform compatibility. It is freely available to academic users at http:\/\/www.structuralchemistry.org\/pcsb\/. To download DMAN, users will be asked for their name, institution and email address. A manual can also be downloaded from this site.<\/jats:p>\n               <jats:p>Contact: \u00a0conan.wang@griffith.edu.au; a.hofmann@griffith.edu.au<\/jats:p>","DOI":"10.1093\/bioinformatics\/btr664","type":"journal-article","created":{"date-parts":[[2011,12,2]],"date-time":"2011-12-02T04:38:17Z","timestamp":1322800697000},"page":"439-440","source":"Crossref","is-referenced-by-count":36,"title":["DMAN: a Java tool for analysis of multi-well differential scanning fluorimetry experiments"],"prefix":"10.1093","volume":"28","author":[{"given":"Conan K.","family":"Wang","sequence":"first","affiliation":[{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saroja K.","family":"Weeratunga","sequence":"additional","affiliation":[{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chris M.","family":"Pacheco","sequence":"additional","affiliation":[{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Hofmann","sequence":"additional","affiliation":[{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"},{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"},{"name":"1 Structural Chemistry Program, Eskitis Institute for Cell and Molecular Therapies, Griffith University, Brisbane, Queensland 4111, 2Faculty of Veterinary Science, The University of Melbourne, Werribee, Victoria 3052 and 3Queensland Tropical Health Alliance"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2011,11,30]]},"reference":[{"key":"2023012512170146700_B1","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/bioinformatics\/18.1.209","article-title":"PCSB - a program collection for structural biology and biophysical chemistry","volume":"18","author":"Hofmann","year":"2002","journal-title":"Bioinformatics"},{"key":"2023012512170146700_B2","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.ab.2004.04.031","article-title":"Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery","volume":"332","author":"Lo","year":"2004","journal-title":"Anal. Biochem."},{"key":"2023012512170146700_B3","doi-asserted-by":"crossref","first-page":"2212","DOI":"10.1038\/nprot.2007.321","article-title":"The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability","volume":"2","author":"Niesen","year":"2007","journal-title":"Nat. Protoc."},{"key":"2023012512170146700_B4","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1177\/108705710100600609","article-title":"High-density miniaturized thermal shift assays as a general strategy for drug discovery","volume":"6","author":"Pantoliano","year":"2001","journal-title":"J. Biomol. Screen."},{"key":"2023012512170146700_B5","doi-asserted-by":"crossref","first-page":"14345","DOI":"10.1021\/bi971719v","article-title":"pH and temperature-induced molten globule-like denatured states of equinatoxin II: a study by UV-melting, DSC, far- and near-UV CD spectroscopy, and ANS fluorescence","volume":"36","author":"Poklar","year":"1997","journal-title":"Biochemistry"},{"key":"2023012512170146700_B6","doi-asserted-by":"crossref","first-page":"15835","DOI":"10.1073\/pnas.0605224103","article-title":"Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination","volume":"103","author":"Vedadi","year":"2006","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"2023012512170146700_B7","first-page":"28","article-title":"The generalization of \u201cStudent\u201d problem when several different population variances are involved","volume":"34","author":"Welch","year":"1947","journal-title":"Biometrika"}],"container-title":["Bioinformatics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/28\/3\/439\/48879070\/bioinformatics_28_3_439.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article-pdf\/28\/3\/439\/48879070\/bioinformatics_28_3_439.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,25]],"date-time":"2023-01-25T14:48:06Z","timestamp":1674658086000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bioinformatics\/article\/28\/3\/439\/188888"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,30]]},"references-count":7,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,2,1]]}},"URL":"https:\/\/doi.org\/10.1093\/bioinformatics\/btr664","relation":{},"ISSN":["1367-4811","1367-4803"],"issn-type":[{"value":"1367-4811","type":"electronic"},{"value":"1367-4803","type":"print"}],"subject":[],"published-other":{"date-parts":[[2012,2,1]]},"published":{"date-parts":[[2011,11,30]]}}}