{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:18:51Z","timestamp":1791245931118,"version":"4.1.0"},"reference-count":13,"publisher":"Oxford University Press (OUP)","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Summary: We present Mytoe, a tool for analyzing mitochondrial morphology and dynamics from fluorescence microscope images. The tool provides automated quantitative analysis of mitochondrial motion by optical flow estimation and of morphology by segmentation of individual branches of the network-like structure of the organelles. Mytoe quantifies several features of individual branches, such as length, tortuosity and speed, and of the macroscopic structure, such as mitochondrial area and degree of clustering. We validate the methods and apply them to the analysis of sequences of images of U2OS human cells with fluorescently labeled mitochondria.<\/jats:p>\n               <jats:p>Availability: Source code, Windows software and Manual available at http:\/\/www.cs.tut.fi\/%7Esanchesr\/mito<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>\n               <jats:p>Contact: \u00a0eero.lihavainen@tut.fi; andre.ribeiro@tut.fi<\/jats:p>","DOI":"10.1093\/bioinformatics\/bts073","type":"journal-article","created":{"date-parts":[[2012,2,10]],"date-time":"2012-02-10T01:22:15Z","timestamp":1328836935000},"page":"1050-1051","source":"Crossref","is-referenced-by-count":58,"title":["Mytoe: automatic analysis of mitochondrial dynamics"],"prefix":"10.1093","volume":"28","author":[{"given":"Eero","family":"Lihavainen","sequence":"first","affiliation":[{"name":"1 Department of Signal Processing, Tampere University of Technology, 2Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland, 3Department of Pediatrics, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarno","family":"M\u00e4kel\u00e4","sequence":"additional","affiliation":[{"name":"1 Department of Signal Processing, Tampere University of Technology, 2Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland, 3Department of Pediatrics, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes N.","family":"Spelbrink","sequence":"additional","affiliation":[{"name":"1 Department of Signal Processing, Tampere University of Technology, 2Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland, 3Department of Pediatrics, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands"},{"name":"1 Department of Signal Processing, Tampere University of Technology, 2Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland, 3Department of Pediatrics, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre S.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"1 Department of Signal Processing, Tampere University of Technology, 2Institute of Biomedical Technology, University of Tampere, FI-33520 Tampere, Finland, 3Department of Pediatrics, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, P.O. 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