{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T17:23:36Z","timestamp":1770485016105,"version":"3.49.0"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"16","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Electrostatic calculations are an important tool for deciphering many functional mechanisms in proteins. Generalized Born (GB) models offer a fast and convenient computational approximation over other implicit solvent-based electrostatic models. Here we present a novel GB-based web server, using the program Bluues, to calculate numerous electrostatic features including pKa-values and surface potentials. The output is organized allowing both experts and beginners to rapidly sift the data. A novel feature of the Bluues server is that it explicitly allows to find electrostatic differences between wild-type and mutant structures.<\/jats:p>\n               <jats:p>Availability: The Bluues server, examples and extensive help files are available for non-commercial use at URL: http:\/\/protein.bio.unipd.it\/bluues\/.<\/jats:p>\n               <jats:p>Contact: \u00a0silvio.tosatto@unipd.it<\/jats:p>","DOI":"10.1093\/bioinformatics\/bts343","type":"journal-article","created":{"date-parts":[[2012,6,19]],"date-time":"2012-06-19T00:19:39Z","timestamp":1340065179000},"page":"2189-2190","source":"Crossref","is-referenced-by-count":78,"title":["Bluues server: electrostatic properties of wild-type and mutated protein structures"],"prefix":"10.1093","volume":"28","author":[{"given":"Ian","family":"Walsh","sequence":"first","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Minervini","sequence":"additional","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Corazza","sequence":"additional","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gennaro","family":"Esposito","sequence":"additional","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvio C. E.","family":"Tosatto","sequence":"additional","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Fogolari","sequence":"additional","affiliation":[{"name":"1 Department of Biology, University of Padova, Viale G. Colombo 3, 35131 Padova, 2Department of Medical and Biolological Sciences, University of Udine, Piazzale Kolbe 4, 33100 Udine and 3Istituto Nazionale Biostrutture e Biosistemi, Viale Medaglie d'Oro 305 - 00136 Roma, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,6,17]]},"reference":[{"key":"2023012512560375100_B1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev.physchem.51.1.129","article-title":"Generalized born models of macromolecular solvation effects","volume":"51","author":"Bashford","year":"2000","journal-title":"Annu. Rev. Phys. Chem."},{"key":"2023012512560375100_B2","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1021\/cr00101a005","article-title":"Electrostatics in biomolecular structure and dynamics","volume":"90","author":"Davis","year":"1990","journal-title":"Chem. 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