{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T04:55:03Z","timestamp":1778907303402,"version":"3.51.4"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"18","license":[{"start":{"date-parts":[[2016,10,2]],"date-time":"2016-10-02T00:00:00Z","timestamp":1475366400000},"content-version":"vor","delay-in-days":1490,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Subcellular localization is one aspect of protein function. Despite advances in high-throughput imaging, localization maps remain incomplete. Several methods accurately predict localization, but many challenges remain to be tackled.<\/jats:p>\n               <jats:p>Results: In this study, we introduced a framework to predict localization in life's three domains, including globular and membrane proteins (3 classes for archaea; 6 for bacteria and 18 for eukaryota). The resulting method, LocTree2, works well even for protein fragments. It uses a hierarchical system of support vector machines that imitates the cascading mechanism of cellular sorting. The method reaches high levels of sustained performance (eukaryota: Q18=65%, bacteria: Q6=84%). LocTree2 also accurately distinguishes membrane and non-membrane proteins. In our hands, it compared favorably with top methods when tested on new data.<\/jats:p>\n               <jats:p>Availability: Online through PredictProtein (predictprotein.org); as standalone version at http:\/\/www.rostlab.org\/services\/loctree2.<\/jats:p>\n               <jats:p>Contact: \u00a0localization@rostlab.org<\/jats:p>\n               <jats:p>Supplementary Information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/bts390","type":"journal-article","created":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T20:35:22Z","timestamp":1347050122000},"page":"i458-i465","source":"Crossref","is-referenced-by-count":98,"title":["LocTree2 predicts localization for all domains of life"],"prefix":"10.1093","volume":"28","author":[{"given":"Tatyana","family":"Goldberg","sequence":"first","affiliation":[{"name":"1 TUM, Bioinformatik-I12, Informatik, Boltzmannstrasse 3, Garching 85748, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Hamp","sequence":"additional","affiliation":[{"name":"1 TUM, Bioinformatik-I12, Informatik, Boltzmannstrasse 3, Garching 85748, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Burkhard","family":"Rost","sequence":"additional","affiliation":[{"name":"1 TUM, Bioinformatik-I12, Informatik, Boltzmannstrasse 3, Garching 85748, Germany"},{"name":"2 New York Consortium on Membrane Protein Structure (NYCOMPS) and Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2012,9,3]]},"reference":[{"key":"2023012513052248100_B1","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1186\/1471-2164-8-78","article-title":"Predicting protein function by machine learning on amino acid sequences\u2014a critical evaluation","volume":"8","author":"Al-Shahib","year":"2007","journal-title":"BMC Genomics"},{"key":"2023012513052248100_B2","doi-asserted-by":"crossref","DOI":"10.1201\/9780203833445","volume-title":"Molecular Biology of the Cell","author":"Alberts","year":"2007"},{"key":"2023012513052248100_B3","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"J. 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