{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T01:43:43Z","timestamp":1782351823638,"version":"3.54.5"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Motivation: Next-generation sequencing affords an efficient analysis of transposon insertion libraries, which can be used to identify essential genes in bacteria. To analyse this high-resolution data, we present a formal Bayesian framework for estimating the posterior probability of essentiality for each gene, using the extreme-value distribution to characterize the statistical significance of the longest region lacking insertions within a gene. We describe a sampling procedure based on the Metropolis\u2013Hastings algorithm to calculate posterior probabilities of essentiality while simultaneously integrating over unknown internal parameters.<\/jats:p>\n               <jats:p>Results: Using a sequence dataset from a transposon library for Mycobacterium tuberculosis, we show that this Bayesian approach predicts essential genes that correspond well with genes shown to be essential in previous studies. Furthermore, we show that by using the extreme-value distribution to characterize genomic regions lacking transposon insertions, this method is capable of identifying essential domains within genes. This approach can be used for analysing transposon libraries in other organisms and augmenting essentiality predictions with statistical confidence scores.<\/jats:p>\n               <jats:p>Availability: A python script implementing the method described is available for download from http:\/\/saclab.tamu.edu\/essentiality\/.<\/jats:p>\n               <jats:p>Contact: \u00a0michael.dejesus@tamu.edu or ioerger@cs.tamu.edu<\/jats:p>\n               <jats:p>Supplementary information: \u00a0Supplementary data are available at Bioinformatics online.<\/jats:p>","DOI":"10.1093\/bioinformatics\/btt043","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T04:44:22Z","timestamp":1359521062000},"page":"695-703","source":"Crossref","is-referenced-by-count":76,"title":["Bayesian analysis of gene essentiality based on sequencing of transposon insertion libraries"],"prefix":"10.1093","volume":"29","author":[{"given":"Michael A.","family":"DeJesus","sequence":"first","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjia J.","family":"Zhang","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M.","family":"Sassetti","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Rubin","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C.","family":"Sacchettini","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Ioerger","sequence":"additional","affiliation":[{"name":"1 Department of Computer Science, Texas A&M University, College Station, TX 77843, 2Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115, 3Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01655 and 4Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,1,29]]},"reference":[{"key":"2023012810285897200_btt043-B1","doi-asserted-by":"crossref","first-page":"8927","DOI":"10.1073\/pnas.95.15.8927","article-title":"Systematic identification of essential genes by in vitro mariner mutagenesis","volume":"95","author":"Akerley","year":"1998","journal-title":"Proc. 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